Western Blot (WB)
(Anti-TNKS rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:142 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human TNKS Polyclonal Antibody | anti-TNKS antibody
Anti-TNKS Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
TNKS; Polyclonal Antibody; Anti-TNKS Antibody; Rabbit Polyclonal; TNKS Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ARTD5 Antibody; Anti-PARP-5a Antibody; Anti-PARP5A Antibody; Anti-PARPL Antibody; Anti-pART5 Antibody; Anti-TIN1 Antibody; Anti-TINF1 Antibody; Anti-TNKS1 Antibody; anti-TNKS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TNKS
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TNKS antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:250-1:1000
IHC-P: 1:250-1:1000
Immunogen
E Coli-derived Human TNKS fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TNKS fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-TNKS rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:142 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-TNKS rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:142 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human TNKS in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human TNKS in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-TNKS antibody
Human tankyrase-1 (TNKS) is a member of the ADP-ribose polymerase (PARP) superfamily of proteins that posttranslationally modify themselves and target proteins with ADP-ribose (termed PARylation). The TNKS ankyrin repeat domain mediates interactions with a number of structurally and functionally diverse binding partners, linking TNKS activity to multiple critical cell processes, including Wnt signaling, Golgi trafficking, and telomere maintenance. The ADP-ribose polymerase Tankyrase (TNKS) mediates proteolysis of Axin-a negative regulator of Wnt signaling-and provides a promising therapeutic target for Wnt-driven diseases. Proteolysis of TNKS substrates is mediated through their ubiquitination by the poly-ADP-ribose (pADPr)-dependent RING-domain E3 ubiquitin ligase RNF146/Iduna. Like TNKS, RNF146 promotes Axin proteolysis and Wnt pathway activation in some cultured cell lines, but in contrast with TNKS, RNF146 is dispensable for Axin degradation in colorectal carcinoma cells. The binding partner:ankyrin repeat domain interface as a viable target for inhibition of TNKS activity.
References
Solberg NT, et al. (2019) Mek inhibition induces canonical wnt signaling through yap in kras mutated hct-15 cells, and a cancer preventive foxo3/foxm1 ratio in combination with tnks inhibition. Cancers (Basel) 11 (2): 164.Solberg NT, et al. (2018) Tankyrase inhibition enhances the antiproliferative effect of pi3k and egfr inhibition, mutually affecting beta-catenin and akt signaling in colorectal cancer. Mol Cancer Res 16 (3): 543-553.Eisemann T, et al. (2019) Structural and functional analysis of parameters governing tankyrase-1 interaction with telomeric repeat-binding factor 1 and gdp-mannose 4, 6-dehydratase. J Biol Chem 294 (40): 14574-14590.
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Product Notes
The TNKS (Catalog #AAA8109873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TNKS Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNKS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 IHC-P: 1:250-1:1000. Researchers should empirically determine the suitability of the TNKS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNKS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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