Western Blot (WB)
(Anti-NEDD9 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:100 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human NEDD9 Polyclonal Antibody | anti-NEDD9 antibody
Anti-NEDD9 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NEDD9; Polyclonal Antibody; Anti-NEDD9 Antibody; Rabbit Polyclonal; NEDD9 Antibody; Rabbit PAb; Antigen Affinity Purified; neural precursor cell expressed; developmentally down-regulated 9; Anti-CAS-L Antibody; Anti-CAS2 Antibody; Anti-CASL Antibody; Anti-CASS2 Antibody; Anti-HEF1 Antibody; anti-NEDD9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NEDD9
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NEDD9 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human NEDD9 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NEDD9 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-NEDD9 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:100 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-NEDD9 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:100 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human NEDD9 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human NEDD9 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-NEDD9 antibody
Scaffold protein neural precursor cell expressed, developmentally downregulated 9 (NEDD9) is a member of the Crk-associated substrate protein family and is known to be a biomarker in multiple cancer types. NEDD9 (also known as CasL and HEF1) encodes a multi-domain scaffolding protein that assembles signaling complexes regulating multiple cellular processes relevant to cancer. These include responsiveness to signals emanating from the T and B cell receptors, integrins, chemokine receptors, and receptor tyrosine kinases, as well as cytoplasmic oncogenes such as BCR-ABL and FAK-and SRC-family kinases. It serves a critical function in regulating cell proliferation, migration, invasion, and survival. NEDD9 plays an integral role in natural and pathological cell biology. Overexpression of NEDD9 protein has been correlated with poor prognosis in various types of cancer.
References
Wang J, et al. (2017) Overexpression of nedd9 in renal cell carcinoma is associated with tumor migration and invasion. Oncol Lett 14 (6): 8021-8027.Shagisultanova E, et al. (2015) Preclinical and clinical studies of the nedd9 scaffold protein in cancer and other diseases. Gene 567 (1): 1-11.Meng H, et al. (2019) Nedd9 promotes invasion and migration of colorectal cancer cell line hct116 via jnk/emt. Oncol Lett 18 (4): 4022-4029.Lu P, et al. (2015) Expression of nedd9 in hepatocellular carcinoma and its clinical significance. Oncol Rep 33 (5): 2375-2383.
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Product Notes
The NEDD9 (Catalog #AAA8108178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NEDD9 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the NEDD9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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