Rabbit TNFRSF1B / TNFR2 / p75 Polyclonal Antibody | anti-TNFRSF1B antibody

Rabbit Polyclonal (IgG) to Mouse TNFRSF1B / TNFR2 / p75

Cat. Num. AAA243925

• Gene Names: TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: TNFRSF1B / TNFR2 / p75; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Mouse TNFRSF1B / TNFR2 / p75; TNFRSF1B Antibody; CD120b antigen Antibody; CD120b Antibody; Etanercept Antibody; p75 TNF receptor Antibody; Soluble TNFR1B variant 1 Antibody; TNFBR Antibody; Tumor necrosis factor receptor Antibody; TNF-R-II Antibody; TNF-R75 Antibody; TNFR2 Antibody; p75TNFR Antibody; p80 TNF-alpha receptor Antibody; TBPII Antibody; TNF-R2 Antibody; TNFR80 Antibody; TNF-RII Antibody; TNFR-II Antibody; TNFR1B Antibody; anti-TNFRSF1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10 mM PBS, pH 7.4, BSA, with less than 0.1% sodium azide.
• Sequence Length: 461

• Applicable Applications for anti-TNFRSF1B antibody: Immunohistochemistry, Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (1:50); ; (Optimal dilution to be determined by the researcher).
• Target Species: Mouse
• Immunogen Description: A synthetic peptide derived from C-terminal of mouse TNF-R2.
• Family/Subfamily: TNF Receptor/not assigned-TNF Receptor.
• Immunogen Type: Synthetic peptide
• Immunogen: TNFRSF1B / TNFR2 antibody was raised against a synthetic peptide derived from C-terminal of mouse TNF-R2.
• Epitope: C-Terminus
• Target: Mouse TNFRSF1B/TNFR2
• Immunogen Species: TNFRSF1B/TNFR2 antibody was raised against Mouse.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TNFR2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Related Product Information for anti-TNFRSF1B antibody

TNFR2 is a 75 kD transmembrane glycoprotein that also exists as a secreted protein. TNF receptors self assemble through a distinct functional extracellular domain termed PLAD in the absence of ligand. Efficient TNFA binding depends on receptor self assembly. TNFR2 has been shown to be responsible for stimulation of activated T lymphocytes by TNFalpha.

Product Categories/Family for anti-TNFRSF1B antibody

Family: TNF Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4507577
• NCBI GeneID: 7133
• NCBI Accession #: NP_001057.1
• NCBI GenBank Nucleotide #: NM_001066.2
• UniProt Accession #: P20333; Q16042; Q6YI29; Q9UIH1; B1AJZ3
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1B
• NCBI Official Symbol: TNFRSF1B
• NCBI Official Synonym Symbols: p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1B; TNF-R2; TNF-RII; p75 TNF receptor; p80 TNF-alpha receptor; soluble TNFR1B variant 1; tumor necrosis factor receptor 2; tumor necrosis factor beta receptor; tumor necrosis factor receptor type II; tumor necrosis factor binding protein 2
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1B
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF1B
• UniProt Synonym Gene Names: TNFBR; TNFR2; TNF-R2; TNF-RII; TNFR-II
• UniProt Entry Name: TNR1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cell soma; perinuclear region of cytoplasm; integral to membrane; extracellular region; plasma membrane; nucleus; lipid raft

Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; immune response; inflammatory response; RNA destabilization; positive regulation of membrane protein ectodomain proteolysis; aging