Rabbit anti-Mouse TNFR2 Polyclonal Antibody | anti-TNFRSF1B antibody

TNFR2, CT (Tumor Necrosis Factor Receptor Superfamily Member 1B, Tumor Necrosis Factor Receptor 2, TNF-R2, Tumor Necrosis Factor Receptor Type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha Receptor, CD120b, Etanercept, TNFRSF1B, TNFBR)

Cat. Num. AAA646657

• Gene Names: TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TNFR2; Polyclonal Antibody; CT (Tumor Necrosis Factor Receptor Superfamily Member 1B; Tumor Necrosis Factor Receptor 2; TNF-R2; Tumor Necrosis Factor Receptor Type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha Receptor; CD120b; Etanercept; TNFRSF1B; TNFBR); Anti -TNFR2; anti-TNFRSF1B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse TNF-R2. Species sequence homology: human and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4.

• Applicable Applications for anti-TNFRSF1B antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1:50; Western Blot: 1-2ug/ml
• Immunogen: Synthetic peptide corresponding to the C-terminal of mouse TNF-R2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-TNFRSF1B antibody

TNFR2 is a 75kD transmembrane glycoprotein that also exists as a secreted protein. TNF receptors self assemble through a distinct functional extracellular domain termed PLAD in the absence of ligand. Efficient TNFA binding depends on receptor self assembly. TNFR2 has been shown to be responsible for stimulation of activated T lymphocytes by TNFalpha.

Product Categories/Family for anti-TNFRSF1B antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4507577
• NCBI GeneID: 7133
• NCBI Accession #: NP_001057.1
• NCBI GenBank Nucleotide #: NM_001066.2
• UniProt Accession #: P20333; Q16042; Q6YI29; Q9UIH1; B1AJZ3
• Molecular Weight: 48,291 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1B
• NCBI Official Symbol: TNFRSF1B
• NCBI Official Synonym Symbols: p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1B; TNF-R2; TNF-RII; p75 TNF receptor; p80 TNF-alpha receptor; soluble TNFR1B variant 1; tumor necrosis factor receptor 2; tumor necrosis factor beta receptor; tumor necrosis factor receptor type II; tumor necrosis factor binding protein 2
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1B
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF1B
• UniProt Synonym Gene Names: TNFBR; TNFR2; TNF-R2; TNF-RII; TNFR-II
• UniProt Entry Name: TNR1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Ref.4 Ref.19

Subunit structure: Binds to TRAF2. Interacts with BMX. Ref.18 Ref.19

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein Ref.4. Isoform 2: Secreted Ref.4. Tumor necrosis factor-binding protein 2: Secreted Ref.4.

Post-translational modification: Phosphorylated; mainly on serine residues and with a very low level on threonine residues.A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

Pharmaceutical use: Available under the name Enbrel (Immunex and Wyeth-Ayerst). Used to treat moderate to severe rheumatoid arthritis (RA). Enbrel consist of the extracellular ligand-binding portion of TNFRSF1B linked to an immunoglobulin Fc chain. It binds to TNF-alpha and blocks its interactions with receptors.

Sequence similarities: Contains 4 TNFR-Cys repeats.