Mouse anti-Human CD40 Monoclonal Antibody | anti-CD40 antibody

Mouse Monoclonal [clone EA-5] (IgG1) to Human CD40

Cat. Num. AAA249012

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Functional Assay
• Purity: Protein A Purified
• Synonyms: CD40; Monoclonal Antibody; Mouse Monoclonal [clone EA-5] (IgG1) to Human CD40; Anti-CD40 Antibody (clone EA-5; Azide-free) IHC-plus; B-cell surface antigen CD40; B cell-associated molecule; B cell surface antigen CD40; CD40 antigen; CD40 type II isoform; p50; Bp50; CD40L receptor; CDW40; TNFRSF5; Human CD40; anti-CD40 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: EA-5
• Specificity: CD40 molecule of about 44 kd.
• Purity/Purification: Protein A Purified
• Form/Format: 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-CD40 antibody: Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS), ELISA (EIA), Functional Assay
• Application Notes: IHC-Paraffin (10ug/ml); Function: Binding of cross linked EA-5 will partially activate B cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
• Family/Subfamily: TNF Receptor/ not assigned-TNF Receptor
• Target Species: Human
• Immunogen Description: Murine L cells transfectants expressing human CD40.
• Immunogen Type: Cells
• Usage: Function: Binding of cross linked EA-5 will partially activate B cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
• Immunogen: CD40 antibody was raised against murine L cells transfectants expressing human CD40.
• Immunogen Species: CD40 antibody was raised against Human
• Antibody Modification: Azide-free
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at +4 degree C. Do not freeze. Open under aseptic conditions.

Immunohistochemistry (IHC)

(Anti-CD40 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.This image was taken for the unmodified form of this product. Other forms have not been tested.)

Related Product Information for anti-CD40 antibody

CD40 is a member of the TNF-receptor superfamily. It has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions.

Product Categories/Family for anti-CD40 antibody

Family: TNF Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4507581
• NCBI GeneID: 958
• NCBI Accession #: NP_001241.1
• NCBI GenBank Nucleotide #: NM_001250.5
• UniProt Accession #: P25942; Q53GN5; Q5JY15; Q5U007; Q7M4Q8; Q86YK5; Q9BYU0; E1P5S9
• Molecular Weight: 22,259 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 5 isoform 1
• NCBI Official Synonym Full Names: CD40 molecule, TNF receptor superfamily member 5
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); nerve growth factor receptor-related B-lymphocyte activation molecule
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: CD40
• UniProt Synonym Gene Names: TNFRSF5
• UniProt Entry Name: TNR5_HUMAN

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; platelet activation; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of B cell proliferation; protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion

Disease: Immunodeficiency With Hyper-igm, Type 3