Western Blot (WB)
(Figure 1 WB Validation in Human Testis Loading: 15 ug of lysate Antibodies: TMPRSS2 9569, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 1ug/mL Lane 2: 2ug/mL Lane 3: 4ug/mL)
Rabbit TMPRSS2 Polyclonal Antibody | anti-TMPRSS2 antibody
TMPRSS2 (CT) Antibody
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
IF: 20ug/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
The immunogen is located within the last 50 amino acids of TMPRSS2.
Western Blot (WB)
(Figure 1 WB Validation in Human Testis Loading: 15 ug of lysate Antibodies: TMPRSS2 9569, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 1ug/mL Lane 2: 2ug/mL Lane 3: 4ug/mL)
Western Blot (WB)
(Figure 1 WB Validation in Human Testis Loading: 15 ug of lysate Antibodies: TMPRSS2 9569, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 1ug/mL Lane 2: 2ug/mL Lane 3: 4ug/mL)
Western Blot (WB)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: TMPRSS2 9569, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Western Blot (WB)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane. Antibodies: TMPRSS2 9569, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Western Blot (WB)
(Figure 3 Western Blot Validation in Rat TissuesLoading: 15 ug of lysates per lane.Antibodies: TMPRSS2, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Western Blot (WB)
(Figure 3 Western Blot Validation in Rat TissuesLoading: 15 ug of lysates per lane.Antibodies: TMPRSS2, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Immunofluorescence (IF)
(Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 5 Immunofluorescence Validation of TMPRSS2 in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 with 9569 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 5 Immunofluorescence Validation of TMPRSS2 in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 with 9569 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse KidneyImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse KidneyImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Immunofluorescence (IF)
(Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Ko et al. Cancer Res. 2015; 75(14):2949-60.
Zang et al. Sci. Immunol. 2020; 5(47):eabc3582.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Uniprot Description
Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.
Research Articles on TMPRSS2
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Product Notes
The TMPRSS2 tmprss2 (Catalog #AAA154821) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TMPRSS2 (CT) Antibody reacts with Human, Mouse, Rat Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%). and may cross-react with other species as described in the data sheet. AAA Biotech's TMPRSS2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). WB: 2ug/mL IF: 20ug/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the TMPRSS2 tmprss2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMPRSS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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