Rabbit TMEM18 Polyclonal Antibody | anti-TMEM18 antibody

TMEM18 Antibody

Cat. Num. AAA151130

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TMEM18 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TMEM18; Polyclonal Antibody; TMEM18 Antibody; Transmembrane protein 18; transmembrane protein 18; anti-TMEM18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TMEM18 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 171

• Applicable Applications for anti-TMEM18 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TMEM18 antibody can be used for detection of TMEM18 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TMEM18 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TMEM18.
• Buffer: TMEM18 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TMEM18 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TMEM18 in rat brain lysate with TMEM18 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TMEM18 in mouse brain tissue with TMEM18 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TMEM18 in Mouse Brain cells with TMEM18 antibody at 20 μg/mL.)

Related Product Information for anti-TMEM18 antibody

TMEM18 Antibody: Recent studies have examined how overexpression of certain genes enhances the tropism of neural stem cells (NSCs) for gliomas. Overexpression of Transmembrane protein 18 (TMEM18) provides NSCs and neural precursors an increased migration capacity towards glioblastoma cells in vitro and in the rat brain, while functional inactivation of the gene resulted in an almost complete loss of migration activity, suggesting that TMEM18 could be a potential target in NSC-based glioma therapy. Other recent results suggest that among others, the TMEM18 loci is associated with higher than normal body mass index (BMI) and obesity, indicating there may be a CNS-related role in predisposition to obesity. Multiple isoforms of TMEM18 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 119621502
• NCBI GeneID: 129787
• UniProt Accession #: Q96B42; Q8N5H2; Q9NTH3; D6W4X9
• Molecular Weight: 15,011 Da
• NCBI Official Full Name: transmembrane protein 18, isoform CRA_a, partial
• NCBI Official Synonym Full Names: transmembrane protein 18
• NCBI Official Symbol: TMEM18
• NCBI Protein Information: transmembrane protein 18
• UniProt Protein Name: Transmembrane protein 18
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMEM18
• UniProt Entry Name: TMM18_HUMAN

Uniprot Description

TMEM18: Transcription repressor. Sequence-specific ssDNA and dsDNA binding protein, with preference for GCT end CTG repeats. Cell migration modulator which enhances the glioma-specific migration ability of neural stem cells (NSC) and neural precursor cells (NPC). Belongs to the TMEM18 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: nuclear membrane; cytoplasm; integral to membrane

Molecular Function: DNA binding

Biological Process: cell migration; transcription, DNA-dependent