Testing Data
(SDS-PAGE analysis of recombinant rat soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)
Podoplanin, soluble Active Protein | Pdpn active protein
Rat Podoplanin, soluble
Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
Avoid freeze/thaw cycles.
Testing Data
(SDS-PAGE analysis of recombinant rat soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)
Testing Data
(SDS-PAGE analysis of recombinant rat soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)
NCBI and Uniprot Product Information
NCBI Description
membrane glycoprotein that may function in lung development [RGD, Feb 2006]
Uniprot Description
Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels
By similarity.
Subcellular location: Membrane; Single-pass type I membrane protein. Cell projection › lamellipodium membrane; Single-pass type I membrane protein. Cell projection › filopodium membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Cell projection › ruffle membrane; Single-pass type I membrane protein. Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. Ref.3
Tissue specificity: In adult kidney, expressed on the urinary surface and foot processes of podocytes and in parietal epithelial cells of Bowman's capsule where it is localized to luminal surfaces. In lung, expressed exclusively on luminal surfaces of type I alveolar epithelial cells and pleural mesothelial cells. Not expressed in type II alveolar cells. In bone, expressed in osteocytes and osteoblasts. In spleen, liver, stomach and intestine, expressed in mesoepithelium. Also expressed in thymic epithelial cells, choroid plexus and leptomeninges. Ref.3 Ref.4
Developmental stage: In newborn kidney, not detected at the vesicle stage. First detected in S-shaped bodies. Ref.3
Post-translational modification: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Ref.6The N-terminus is blocked.
Miscellaneous: Down-regulated in puromycin aminonucleoside nephrosis (PAN).
Sequence similarities: Belongs to the podoplanin family.
Research Articles on Pdpn
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Product Notes
The Pdpn pdpn (Catalog #AAA692151) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Rat Podoplanin, soluble reacts with Rat and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: GAIGALEDDL VTPGPGDDMV NPGLEDRIET TDTTGELDKS TAKAPLVPTQ PPIEELPTSG TSDHDHKEHE STTTVKAVTS HSTDKKTTHP NRDNAGGETQ TTDKKDGLAV VTLEHHHHHH. It is sometimes possible for the material contained within the vial of "Podoplanin, soluble, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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