Rabbit anti-Human, Mouse TLR2 Polyclonal Antibody | anti-TLR2 antibody

Rabbit Polyclonal to Human TLR2

Cat. Num. AAA241331

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Ion exchange Chromatography
• Synonyms: TLR2; Polyclonal Antibody; Rabbit Polyclonal to Human TLR2; Anti-TLR2 Antibody (N-Terminus) IHC-plus; CD282 antigen; CD282; TIL4; Toll-like receptor 2; Human TLR2; anti-TLR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: peptide corresponding to 14 amino acids near the amino terminus of human TLR2
• Purity/Purification: Ion exchange Chromatography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TLR2.
• Immunogen Type: Synthetic peptide
• Immunogen: TLR2 antibody was raised against synthetic peptide from human TLR2.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-TLR2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TLR2 in Human Spleen cells with TLR2 antibody at 10 ug/ml.)

Western Blot (WB)

(Western blot of TLR2 in A20 cell lysates with TLR2 antibody at 1 ug/ml in the presence (A) and absence (B) of its blocking peptide.)

Related Product Information for anti-TLR2 antibody

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kB.

Product Categories/Family for anti-TLR2 antibody

Family: Toll-like Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 19718734
• NCBI GeneID: 7097
• NCBI Accession #: NP_003255.2
• NCBI GenBank Nucleotide #: NM_003264.3
• UniProt Accession #: O60603; O15454; Q8NI00; B3Y612; D1CS45; D1CS48; D1CS49
• Molecular Weight: 89,838 Da
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Cell surface; Apoptosis

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell projection; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; protein heterodimerization activity; triacylated lipoprotein binding; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; response to toxin; positive regulation of leukocyte migration; leukotriene metabolic process; positive regulation of NF-kappaB import into nucleus; detection of triacylated bacterial lipoprotein; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; toll-like receptor 4 signaling pathway; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; defense response to Gram-positive bacterium; myelin formation in the central nervous system; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; apoptosis; positive regulation of interleukin-12 production; microglial cell activation; response to molecule of fungal origin; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; induction by symbiont of defense-related host nitric oxide production; negative regulation of interleukin-17 production; toll-like receptor signaling pathway; response to hypoxia; innate immune response; immune response; chloramphenicol transport; positive regulation of inflammatory response; positive regulation of cytokine secretion

Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To