Rabbit anti-Human, Mouse TLR2 Polyclonal Antibody | anti-TLR2 antibody

TLR2 Antibody

Cat. Num. AAA150600

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TLR2 Antibody is Ion exchange chromatography purified.
• Synonyms: TLR2; Polyclonal Antibody; TLR2 Antibody; TIL4; CD282; Toll-like receptor 2; toll-like receptor 2; anti-TLR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TLR2 Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TLR2 antibody can be used for detection of TLR2 by Western blot at 1 ?g/mL. Despite itspredicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibodycan also detect TLR2 by immunohistochemistry at 2 ?g/mL. For immunofluorescence startat 10 ?g/mL.; ; Antibody validated: Western Blot in mouse samples; Immunohistochemistry in humansamples and Immunofluorescence in human samples. All other applications and speciesnot yet tested.
• Conjugate: Unconjugated
• Immunogen: TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2.; ; The immunogen is located within the first 50 amino acids of TLR2.
• Buffer: TLR2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TLR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TLR2 in A20 cell lysates with TLR2 antibody at 1 μg/mL in the presence (A) and absence (B) of its blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human spleen cells using TLR2 antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TLR2 in Human Spleen tissue with TLR2 antibody at 10 μg/mL.)

Related Product Information for anti-TLR2 antibody

TLR2 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer can recognize a variety of bacterial and mycoplasma lipoproteins respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 20140434
• NCBI GeneID: 7097
• UniProt Accession #: O60603; O15454; Q8NI00; B3Y612; D1CS45; D1CS48; D1CS49
• NCBI Official Full Name: Toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Cell surface; Motility/polarity/chemotaxis; Apoptosis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell projection; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; triacylated lipoprotein binding; protein heterodimerization activity; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; response to toxin; positive regulation of leukocyte migration; leukotriene metabolic process; positive regulation of NF-kappaB import into nucleus; detection of triacylated bacterial lipoprotein; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; toll-like receptor 4 signaling pathway; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; defense response to Gram-positive bacterium; myelin formation in the central nervous system; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; apoptosis; positive regulation of interleukin-12 production; microglial cell activation; response to molecule of fungal origin; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of toll-like receptor signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; negative regulation of interleukin-17 production; toll-like receptor signaling pathway; response to hypoxia; innate immune response; immune response; chloramphenicol transport; positive regulation of inflammatory response; positive regulation of cytokine secretion

Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To