Rabbit anti-Human, Mouse Toll-like receptor 2 (TLR2) Polyclonal Antibody | anti-TLR2 antibody

Toll-like receptor 2 (TLR2) (NT)

Cat. Num. AAA395815

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: Toll-like receptor 2 (TLR2); Polyclonal Antibody; Toll-like receptor 2 (TLR2) (NT); TIRAP (CT); MAL; TIR domain containing adaptor protein; anti-TLR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as solution in phosphate buffered saline with 0.02% sodium azide
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Rabbit polyclonal TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2.
• Positive Control: Located in the membrane, TLR2 is highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.8
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis of TLR2 in A20 cell lysates with TLR2 antibody at 1 g/ml in the presence (A) and absence (B) of its blocking peptide)

Related Product Information for anti-TLR2 antibody

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) α/β and NF-κB (4-6). TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer can recognize a variety of bacterial and mycoplasma lipoproteins respectively (reviewed in 7).

Product Categories/Family for anti-TLR2 antibody

Polyclonal Antibody; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 19718734
• NCBI GeneID: 7097
• NCBI Accession #: NP_003255.2
• NCBI GenBank Nucleotide #: NM_003264.3
• UniProt Accession #: O60603; O15454; Q8NI00; B3Y612; D1CS45; D1CS48; D1CS49
• Molecular Weight: 89.8 kD
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2; toll/interleukin 1 receptor-like 4; toll/interleukin-1 receptor-like protein 4
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

Subunit structure: Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1 or TLR6. Interacts with CNPY3

By similarity. Interacts with ATG16L1. Ref.10 Ref.12

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

Domain: Ester-bound lipid substrates are bound through a crevice formed between the LRR 11 and LRR 12

By similarity.The ATG16L1-binding motif mediates interaction with ATG16L1 (Ref.12).

Post-translational modification: Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

Polymorphism: Genetic variations in TLR2 are associated with susceptibility to leprosy [

MIM:246300]. Leprosy is a chronic disease associated with depressed cellular (but not humoral) immunity, the bacterium requires a lower temperature than 37 degrees Celsius and thrives particularly in peripheral Schwann cells and macrophages. The Trp-677 polymorphism in the intracellular domain of TLR2 has a role in susceptibility to lepromatous leprosy. Wild-type TLR2 mediates CD14-enhanced Mycobacterium leprae-dependent activation of NFKB1, but TLR2 containing the Trp-677 polymorphism did not. The impaired function of the Trp-677 polymorphism provides a molecular mechanism for the poor cellular immune response associated with lepromatous leprosy.

Sequence similarities: Belongs to the Toll-like receptor family.Contains 19 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.Contains 1 TIR domain.