Dot Blot (DB)
(Dot blot analysis of Phospho-FOS-T232 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Rabbit anti-Human FOS Polyclonal Antibody | anti-FOS antibody
FOS, phosphorylated (Thr232) (Proto-oncogene c-Fos, Cellular Oncogene Fos, G0/G1 Switch Regulatory Protein 7, G0S7)
Gene Names
FOS; AP-1; C-FOS
Reactivity
Human
Applications
ELISA, Western Blot, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
FOS; Polyclonal Antibody; phosphorylated (Thr232) (Proto-oncogene c-Fos; Cellular Oncogene Fos; G0/G1 Switch Regulatory Protein 7; G0S7); Anti -FOS; anti-FOS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human FOS when phosphorylated at Thr232..
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-FOS antibody
ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA and Dot Blot.
Dilution: ELISA: 1:1,000
Dot Blot: 1:100-1:500
Dilution: ELISA: 1:1,000
Dot Blot: 1:100-1:500
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr232 of human FOS (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of Phospho-FOS-T232 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Dot Blot (DB)
(Dot blot analysis of Phospho-FOS-T232 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)
Product Categories/Family for anti-FOS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
40,695 Da
NCBI Official Full Name
FOS
NCBI Official Synonym Full Names
FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Symbol
FOS
NCBI Official Synonym Symbols
AP-1; C-FOS
NCBI Protein Information
proto-oncogene c-Fos; activator protein 1; cellular oncogene fos; cellular oncogene c-fos; G0/G1 switch regulatory protein 7; v-fos FBJ murine osteosarcoma viral oncogene homolog; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
UniProt Protein Name
FOS protein
Protein Family
UniProt Gene Name
FOS
UniProt Entry Name
Q6FG41_HUMAN
NCBI Description
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq]
Uniprot Description
Sequence similarities: Belongs to the bZIP family. RuleBase RU000470Contains 1 bZIP domain. SAAS SAAS011616
Research Articles on FOS
Similar Products
Product Notes
The FOS fos (Catalog #AAA625338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOS, phosphorylated (Thr232) (Proto-oncogene c-Fos, Cellular Oncogene Fos, G0/G1 Switch Regulatory Protein 7, G0S7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOS can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA and Dot Blot. Dilution: ELISA: 1:1,000 Dot Blot: 1:100-1:500. Researchers should empirically determine the suitability of the FOS fos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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