Rabbit anti-Human C10orf66 Polyclonal Antibody | anti-C10orf66 antibody

C10orf66, CT (CUE Domain-containing Protein 2, HOYS6, CUEDC2) (PE)

Cat. Num. AAA6469009

• Gene Names: CUEDC2; C10orf66; bA18I14.5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: C10orf66; Polyclonal Antibody; CT (CUE Domain-containing Protein 2; HOYS6; CUEDC2) (PE); anti-C10orf66 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CUEDC2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-C10orf66 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CUEDC2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CUEDC2 Antibody (C-term) in Y79 cell line lysates (35ug/lane). CUEDC2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain with CUEDC2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using CUEDC2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-C10orf66 antibody

Antibodies; Nuclear Proteins

References

1. Grupe, A., et.al., Am. J. Hum. Genet. 78(1), 78-88 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 148596996
• NCBI GeneID: 79004
• NCBI Accession #: NP_076945
• NCBI GenBank Nucleotide #: NM_024040.2
• UniProt Accession #: Q9H467; Q9BWG8; D3DR88
• Molecular Weight: 32,009 Da
• NCBI Official Full Name: CUE domain-containing protein 2
• NCBI Official Synonym Full Names: CUE domain containing 2
• NCBI Official Symbol: CUEDC2
• NCBI Official Synonym Symbols: C10orf66; bA18I14.5
• NCBI Protein Information: CUE domain-containing protein 2
• UniProt Protein Name: CUE domain-containing protein 2
• UniProt Gene Name: CUEDC2
• UniProt Synonym Gene Names: C10orf66
• UniProt Entry Name: CUED2_HUMAN

Uniprot Description

CUEDC2: Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism. May predict the clinical outcome of tamoxifen therapy of breast cancer patients. Patients with tumors that highly express CUEDC2 do not respond to tamoxifen treatment as effectively as those with tumors with low expression. Belongs to the CUEDC2 family.

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm

Molecular Function: protein binding