Rabbit KLF10/KLF11 Polyclonal Antibody | anti-KLF10 antibody

KLF10/KLF11 Polyclonal Antibody

Cat. Num. AAA3007194

• Gene Names: KLF10; EGRA; TIEG; TIEG1; EGR-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: KLF10/KLF11; Polyclonal Antibody; KLF10/KLF11 Polyclonal Antibody; Krueppel-like factor 10; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1; TGFB-inducible early growth response protein 1; TIEG-1; KLF10; TIEG; TIEG1; anti-KLF10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KLF10/KLF11 polyclonal antibody detects endogenous levels of KLF10 and KLF11 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 469

• Applicable Applications for anti-KLF10 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human KLF10/11.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of KLF10/KLF11 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-KLF10 antibody

Originally isolated from osteoblastic cells, the TGFbeta1-inducible early gene-1 (TIEG1) is a Krupel-like zinc finger transcription factor-encoding gene which regulates cellular growth and differentiation. TIEG1 is regulated as an early response gene by TGFbeta1. It is expressed in both acinar and ductular epithelial cells from exocrine pancreas and may serve as an early response gene in pancreatic cell lines. Further, overexpression of TIEG1 in TGFbeta-sensitive epithelial cells induces apoptosis. TIEG1 and EGRa are expressed from alternate promoters of the same gene. Both are highly expressed in human fetal osteoblast cells. TIEG1 is additionally expressed at high levels in PBLs, spleen and colon, and at lower levels in thymus, small intestine, ovary, prostate and skeletal muscle. The nuclear TIEG2 protein, which shares significant homology with TIEG1, was originally isolated from globin-expressing human fetal erythroid cells. TIEG2 is also expressed in fetal liver. Overexpression of TIEG2 in cultured epithelial cells inhibits cellular proliferation. TIEG2 expression is upregulated by TGFbeta1 and serum deprivation.

NCBI and Uniprot Product Information

• NCBI GI #: 73760403
• NCBI GeneID: 7071
• NCBI Accession #: NP_001027453
• NCBI GenBank Nucleotide #: NP_001027453.1
• UniProt Accession #: Q13118; O14901
• Molecular Weight: ~ 14kDa
• NCBI Official Full Name: Krueppel-like factor 10 isoform b
• NCBI Official Synonym Full Names: Kruppel like factor 10
• NCBI Official Symbol: KLF10
• NCBI Official Synonym Symbols: EGRA; TIEG; TIEG1; EGR-alpha
• NCBI Protein Information: Krueppel-like factor 10
• UniProt Protein Name: Krueppel-like factor 10
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF10
• UniProt Synonym Gene Names: TIEG; TIEG1; TGFB-inducible early growth response protein 1; TIEG-1
• UniProt Entry Name: KLF10_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]