Rabbit anti-Human, Mouse TFEB Polyclonal Antibody | anti-TFEB antibody

TFEB Polyclonal Antibody

Cat. Num. AAA9707201

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TFEB; Polyclonal Antibody; TFEB Polyclonal Antibody; Rabbit Anti-TFEB Polyclonal Antibody; BHLHE35; Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35; anti-TFEB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TFEB Polyclonal Antibody detects endogenous levels of TFEB protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 490

• Applicable Applications for anti-TFEB antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:300; ELISA: 1:20000
• Immunogen: Synthesized peptide derived from the N-terminal region of human TFEB at AA range: 10-90
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Western Blot (WB)

(Fig.1. Western Blot analysis of Mouse-lung Mouse-muscle cells using TFEB Polyclonal Antibody diluted at 1:500.)

Western Blot (WB)

(Fig.2. Western blot analysis of Mouse-kidney lysis using TFEB antibody. Antibody was diluted at 1:500.)

Related Product Information for anti-TFEB antibody

Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network. TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the up-regulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy. TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in up-regulation of autophagosomes and lysosomes. Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress. During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes. TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB.

NCBI and Uniprot Product Information

• NCBI GI #: 429836865
• NCBI GeneID: 7942
• NCBI Accession #: NP_001161299.2
• NCBI GenBank Nucleotide #: NP_001161299.2
• UniProt Accession #: P19484
• NCBI Official Full Name: transcription factor EB isoform 2
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB
• UniProt Protein Name: Transcription factor EB
• Protein Family: Transcription factor
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN

Uniprot Description

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: embryonic placenta development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; humoral immune response; positive regulation of autophagy