CD5 / LEU1 recombinant protein

Recombinant Mouse CD5 / LEU1 Protein (His tag)

Cat. Num. AAA2547000

• Gene Names: Cd5; Ly-1; Ly-A; Ly-12; Lyt-1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD5 / LEU1; Recombinant Mouse CD5 / LEU1 Protein (His tag); Cd5; Ly-1; Ly-12; Ly-A; Lyt-1; CD5 / LEU1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 494

• Application Notes: The secreted recombinant mouse CD5 consists of 359 amino acids and has a calculated molecular mass of 39.4 kDa. As a result of glycosylation, the apparent molecular mass of rmCD5 is approximately 42-48 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD5 / LEU1 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.

Description: A DNA sequence encoding the extracellular domain of mouse CD5 (NP_031676.3) (Met 1-Pro 371) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 126517489
• NCBI GeneID: 12507
• NCBI Accession #: NP_031676.3
• NCBI GenBank Nucleotide #: NM_007650.3
• UniProt Accession #: P13379
• Molecular Weight: 53,849 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: CD5 antigen
• NCBI Official Symbol: Cd5
• NCBI Official Synonym Symbols: Ly-1; Ly-A; Ly-12; Lyt-1
• NCBI Protein Information: T-cell surface glycoprotein CD5
• UniProt Protein Name: T-cell surface glycoprotein CD5
• UniProt Gene Name: Cd5
• UniProt Synonym Gene Names: Ly-1; Ly-1; Lyt-1

Uniprot Description

CD5: a protein that in lymphocytes is involved in host defences and immune responses. Expressed in T cells, B cells, and chronic lymphocytic leukemia B cells (BCLL). Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. B cells and T cells express a full length isoform expressed on the plasma membrane, while B cells also express a truncated intracellular isoform. B cells expressing the integral membrane isoform are CD5+ by FACS analysis, while those expressing only the cytoplasmic isoform are CD5-. Expression of either isoform mediates the activation of STAT3 and NFAT2 in CLL B and the resultant constitutive production of IL-10, a B cell growth factor. Contains 3 SRCR domains. Two alternatively human isoforms have been reported.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19 A|19 7.16 cM

Cellular Component: external side of plasma membrane; plasma membrane

Molecular Function: glycoprotein binding; protein binding

Biological Process: T cell costimulation