Rabbit anti-Human Mitogen Activated Protein Kinase Activated Protein Kinase 3 Polyclonal Antibody | anti-MAPKAPK3 antibody

Polyclonal Antibody to Mitogen Activated Protein Kinase Activated Protein Kinase 3 (MAPKAPK3)

Cat. Num. AAA2028648

• Gene Names: MAPKAPK3; 3PK; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Mitogen Activated Protein Kinase Activated Protein Kinase 3; Polyclonal Antibody; Polyclonal Antibody to Mitogen Activated Protein Kinase Activated Protein Kinase 3 (MAPKAPK3); anti-MAPKAPK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MAPKAPK3. It has beenselected for its ability to recognize MAPKAPK3 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RI QERGDQAFTE REAAEIMRDI GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD AVLKLTDFGF AKETTQNALQ TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP PFYSNTGQAI SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN HPWINQSMVV PQTPLHTARV LQEDKDHWD

• Applicable Applications for anti-MAPKAPK3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.2-2ug/mL;1:250-2500; Immunohistochemistry: 5-20ug/mL;1:25-100; Immunocytochemistry: 5-20ug/mL;1:25-100; Optimal working dilutions must be determined by end user.
• Fragment: MAPKAPK3 (Arg129~Asp329)
• Organism Species: Homo sapiens (Human)
• Cross Reactivity: Human
• Conjugate: No Conjugate
• Immunogen: Recombinant MAPKAPK3 (Arg129~Asp329) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2057316
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Heart tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 345441756
• NCBI GeneID: 7867
• NCBI Accession #: NP_001230854.1
• NCBI GenBank Nucleotide #: NM_001243925.1
• UniProt Accession #: Q16644; B5BU67
• Molecular Weight: 42,987 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 3
• NCBI Official Symbol: MAPKAPK3
• NCBI Official Synonym Symbols: 3PK; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• NCBI Protein Information: MAP kinase-activated protein kinase 3
• UniProt Protein Name: MAP kinase-activated protein kinase 3
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK3
• UniProt Synonym Gene Names: MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; 3pK

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

MAPKAPK3: a member of the MAPKAPK family of protein kinases. Activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Protein type: CAMK group; EC 2.7.11.1; Kinase, protein; MAPKAPK family; MAPKAPK subfamily; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; MAP kinase kinase activity; mitogen-activated protein kinase binding; protein binding; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; cell surface receptor signaling pathway; macropinocytosis; MAPK cascade; peptidyl-serine phosphorylation; protein autophosphorylation; Ras protein signal transduction; response to cytokine; response to lipopolysaccharide; response to stress; signal transduction; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway

Disease: Macular Dystrophy, Patterned, 3