Rabbit TCF-1 Polyclonal Antibody | anti-TCF-1 antibody

TCF-1 (F26) Polyclonal Antibody

Cat. Num. AAA3001996

• Gene Names: TCF7; TCF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TCF-1; Polyclonal Antibody; TCF-1 (F26) Polyclonal Antibody; Transcription factor 7; TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF7; TCF1; anti-TCF-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TCF-1 (F26) polyclonal antibody detects endogenous levels of TCF-1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 269

• Applicable Applications for anti-TCF-1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human TCF-1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TCF-1 (F26) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:MEF whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TCF-1 (F26) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-TCF-1 antibody

T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic beta-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The beta-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates beta-catenin and, in turn, increases the degradation of cytosolic beta-catenin and inhibits the transcriptional activity of the TCF proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 293651582
• NCBI GeneID: 6932
• NCBI Accession #: NP_001128323.2
• NCBI GenBank Nucleotide #: NP_001128323.2
• UniProt Accession #: P36402
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: transcription factor 7 isoform 3
• NCBI Official Synonym Full Names: transcription factor 7
• NCBI Official Symbol: TCF7
• NCBI Official Synonym Symbols: TCF-1
• NCBI Protein Information: transcription factor 7
• UniProt Protein Name: Transcription factor 7
• UniProt Gene Name: TCF7
• UniProt Synonym Gene Names: TCF1; TCF-7; T-cell factor 1; TCF-1
• UniProt Entry Name: TCF7_HUMAN

NCBI Description

This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]

Uniprot Description

TCF7: Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4. By TCF7L2 and CTNNB1. Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. Belongs to the TCF/LEF family. 16 isoforms of the human protein are produced by alternative promoter.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; beta-catenin binding; chromatin binding; transcription factor activity

Biological Process: neural tube development; transcription, DNA-dependent; alpha-beta T cell differentiation; Wnt receptor signaling pathway through beta-catenin; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; embryonic digestive tract morphogenesis; generation of neurons; T cell receptor V(D)J recombination; immune response; brain development; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis