Rabbit Cadherin-8 Polyclonal Antibody | anti-A519 antibody

Cadherin-8 (A519) Polyclonal Antibody

Cat. Num. AAA3002801

• Gene Names: CDH8; Nbla04261
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cadherin-8; Polyclonal Antibody; Cadherin-8 (A519) Polyclonal Antibody; Cadherin 8; CADH8; Cadherin8; anti-A519 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cadherin-8 (A519) polyclonal antibody detects endogenous levels of Cadherin-8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 799

• Applicable Applications for anti-A519 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human Cadherin-8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cadherin-8 (A519) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cadherin-8 (A519) pAb in paraffin-embedded human brain tissue.)

Western Blot (WB)

(Western Blot (WB) analysis of Cadherin-8 (A519) pAb at 1:500 dilutionLane1:BV2 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of Cadherin-8 (A519) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:The brain tissue lysate of Fetal Mouse (40ug) Lane4:The brain tissue lysate of Fetal Rat (40ug) Lane5:C6 whole cell lysate (40ug))

Related Product Information for anti-A519 antibody

The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. Cadherin-8 expression has been seen in mouse thymus tissue as well as in specific subdivisions of the developing central nervous system. It plays a potential role in brain morphology.

NCBI and Uniprot Product Information

• NCBI GI #: 16306539
• NCBI GeneID: 1006
• NCBI Accession #: NP_001787.2
• NCBI GenBank Nucleotide #: NP_001787.2
• UniProt Accession #: P55286
• Molecular Weight: ~ 88kDa
• NCBI Official Full Name: cadherin-8 preproprotein
• NCBI Official Synonym Full Names: cadherin 8
• NCBI Official Symbol: CDH8
• NCBI Official Synonym Symbols: Nbla04261
• NCBI Protein Information: cadherin-8
• UniProt Protein Name: Cadherin-8
• Protein Family: Cadherin
• UniProt Gene Name: CDH8
• UniProt Entry Name: CADH8_HUMAN

NCBI Description

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH8: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: plasma membrane; integral to membrane; nerve terminal

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; synaptic transmission, glutamatergic; response to cold; cell adhesion; homophilic cell adhesion