Western Blot (WB)
(Western Blot (WB) analysis of TCF-4/12 (S616) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug) Lane6:AML-12 whole cell lysate (40ug))
Rabbit TCF-4/12 Polyclonal Antibody | anti-TCF-4/12 antibody
TCF-4/12 (S616) Polyclonal Antibody
Gene Names
TCF4; E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
TCF-4/12; Polyclonal Antibody; TCF-4/12 (S616) Polyclonal Antibody; Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2; TCF4; BHLHB19; ITF2; SEF2; Transcription factor 12; TCF-12; Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4; TCF12; BHLHB20; HEB; HTF4; anti-TCF-4/12 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TCF-4/12 (S616) polyclonal antibody detects endogenous levels of TCF-4, and also detects TCF-12 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
671
Applicable Applications for anti-TCF-4/12 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 580-630 of Human TCF-4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of TCF-4/12 (S616) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug) Lane6:AML-12 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of TCF-4/12 (S616) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug) Lane6:AML-12 whole cell lysate (40ug))
Related Product Information for anti-TCF-4/12 antibody
T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to beta-catenin in a cooperative manner. This TCF-4/beta-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~ 60, 72kDa
NCBI Official Full Name
transcription factor 4 isoform a
NCBI Official Synonym Full Names
transcription factor 4
NCBI Official Symbol
TCF4
NCBI Official Synonym Symbols
E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
NCBI Protein Information
transcription factor 4
UniProt Protein Name
Transcription factor 4
UniProt Gene Name
TCF4
UniProt Synonym Gene Names
BHLHB19; ITF2; SEF2; TCF-4; bHLHb19; ITF-2; SEF-2
UniProt Entry Name
ITF2_HUMAN
NCBI Description
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016]
Research Articles on TCF-4/12
Similar Products
Product Notes
The TCF-4/12 tcf4 (Catalog #AAA3001901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF-4/12 (S616) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCF-4/12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TCF-4/12 tcf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF-4/12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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