Rabbit anti-Human TAF1B Polyclonal Antibody | anti-TAF1B antibody

TAF1B (TATA Box-binding Protein-associated Factor RNA Polymerase I Subunit B, RNA Polymerase I-specific TBP-associated Factor 63 kDa, TAFI63, TATA Box-binding Protein-associated Factor 1B, TBP-associated Factor 1B, Transcription Initiation Factor SL1/TIF-

Cat. Num. AAA647445

• Gene Names: TAF1B; SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TAF1B; Polyclonal Antibody; TAF1B (TATA Box-binding Protein-associated Factor RNA Polymerase I Subunit B; RNA Polymerase I-specific TBP-associated Factor 63 kDa; TAFI63; TATA Box-binding Protein-associated Factor 1B; TBP-associated Factor 1B; Transcription Initiation Factor SL1/TIF-; Anti -TAF1B (TATA Box-binding Protein-associated Factor RNA Polymerase I Subunit B; anti-TAF1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAF1B.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIAKMAKAVKYDVQAVAIIVVVLKLLFLLDDSFEWSLSNLAEKHNEKNKKDKPWFDFRKWYQIMKKAFDEKKQKWEEARAKYLWKSEKPLYYSFVDKPVAYKKREMVVNLQKQFSTLVDSTATAGKKSPSSFQFNWTEEDTDRTCFHGHSLQGVLKEKGQSLLTKNSLYWLSTQKFCRCYCTHVTTYEESNYSLSYQFILNLFSFLLRIKTSLLHEEVSLVEKKLFEKKYSVKRKKSRSKKVRRH

• Applicable Applications for anti-TAF1B antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human TAF1B, aa1-588 (AAH18137.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TAF1B expression in transfected 293T cell line by TAF1B polyclonal antibody. Lane 1: TAF1B transfected lysate (68.8kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TAF1B antibody

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.

Product Categories/Family for anti-TAF1B antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 205360987
• NCBI GeneID: 9014
• NCBI Accession #: NP_005671.2
• NCBI GenBank Nucleotide #: NM_005680.2
• UniProt Accession #: Q53T94; Q15574; Q8WVC3; B4DI42; F8WD72
• Molecular Weight: 68,832 Da
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit B
• NCBI Official Synonym Full Names: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
• NCBI Official Symbol: TAF1B
• NCBI Official Synonym Symbols: SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit B; SL1, 63kD subunit; TBP-associated factor 1B; TATA box-binding protein-associated factor 1B; transcription initiation factor SL1/TIF-IB subunit B; RNA polymerase I-specific TBP-associated factor 63 kDa
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF1B
• UniProt Synonym Gene Names: TAFI63
• UniProt Entry Name: TAF1B_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes one of the SL1-specific TAFs. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3. Ref.1 Ref.5 Ref.6 Ref.9 Ref.11 Ref.12

Subunit structure: Interacts with FLNA (via N-terminus)

By similarity. Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3. Ref.1 Ref.8

Subcellular location: Nucleus › nucleolus.

Domain: Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides (Ref.11 and Ref.12).

Sequence similarities: Belongs to the RRN7/TAF1B family.Contains 1 RRN7-type zinc finger.

Sequence caution: The sequence AAA62863.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.