Rabbit TAF1B Polyclonal Antibody | anti-TAF1B antibody

TAF1B Antibody (Center)

Cat. Num. AAA9206255

• Gene Names: TAF1B; SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TAF1B; Polyclonal Antibody; TAF1B Antibody (Center); TATA box-binding protein-associated factor RNA polymerase I subunit B; RNA polymerase I-specific TBP-associated factor 63 kDa; TAFI63; TATA box-binding protein-associated factor 1B; TBP-associated factor 1B; Transcription initiation factor SL1/TIF-IB subunit B; anti-TAF1B antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TAF1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 367-396 amino acids from the Central region of human TAF1B.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 367-396
• Sequence Length: 333

• Applicable Applications for anti-TAF1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(TAF1B Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TAF1B antibody detected the TAF1B protein (arrow).)

Related Product Information for anti-TAF1B antibody

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.

NCBI and Uniprot Product Information

• NCBI GI #: 205360987
• NCBI GeneID: 9014
• NCBI Accession #: NP_005671.2
• NCBI GenBank Nucleotide #: NM_005680.2
• UniProt Accession #: Q53T94; Q15574; Q8WVC3; B4DI42; F8WD72
• Molecular Weight: 68832
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit B
• NCBI Official Synonym Full Names: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
• NCBI Official Symbol: TAF1B
• NCBI Official Synonym Symbols: SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit B
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF1B
• UniProt Synonym Gene Names: TAFI63; TBP-associated factor 1B
• UniProt Entry Name: TAF1B_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes one of the SL1-specific TAFs. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF1B: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3. Belongs to the RRN7/TAF1B family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription initiation complex; Nucleolus

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; TATA-binding protein binding; metal ion binding; transcription factor activity

Biological Process: chromatin silencing at rDNA; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic