Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Troponin T, fast skeletal muscle (TNNT3) Standard Curve.)
Mouse Troponin T, Fast Skeletal Muscle (TNNT3) ELISA Kit | TNNT3 elisa kit
Mouse Troponin T, Fast Skeletal Muscle (TNNT3) ELISA Kit
Gene Names
Tnnt3; fTnT
Reactivity
Mouse
Synonyms
Troponin T; Fast Skeletal Muscle (TNNT3); Mouse Troponin T; Fast Skeletal Muscle (TNNT3) ELISA Kit; TNNT3; DKFZp779M2348; TNTF; OTTHUMP00000014435; troponin T; fast skeletal muscle; troponin T3; skeletal; fast; troponin-T3; TNNT3 elisa kit
Reactivity
Mouse
Specificity
Mouse Troponin T, fast skeletal muscle (TNNT3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNT3. No significant cross-reactivity or interference between Mouse TNNT3 and analogues was observed.
Sequence Length
272
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Troponin T, fast skeletal muscle (TNNT3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Troponin T, fast skeletal muscle (TNNT3) Standard Curve.)
Related Product Information for TNNT3 elisa kit
Description: This Mouse Troponin T, fast skeletal muscle (TNNT3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNT3 in samples. An antibody specific for TNNT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
Background: Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
troponin T, fast skeletal muscle isoform 2
NCBI Official Synonym Full Names
troponin T3, skeletal, fast
NCBI Official Symbol
Tnnt3
NCBI Official Synonym Symbols
fTnT
NCBI Protein Information
troponin T, fast skeletal muscle
UniProt Protein Name
Troponin T, fast skeletal muscle
Protein Family
UniProt Gene Name
Tnnt3
UniProt Synonym Gene Names
TnTf; fTnT
UniProt Entry Name
TNNT3_MOUSE
Uniprot Description
TNNT3: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin T family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Motor
Cellular Component: troponin complex
Molecular Function: troponin C binding; troponin I binding; tropomyosin binding; actin binding; calcium-dependent protein binding; calcium-dependent ATPase activity
Biological Process: skeletal muscle contraction; regulation of ATPase activity; metabolic process; regulation of muscle contraction; regulation of striated muscle contraction
Research Articles on TNNT3
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Product Notes
The Mouse TNNT3 tnnt3 (Catalog #AAA9716707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716707 ELISA Kit recognizes Mouse TNNT3. It is sometimes possible for the material contained within the vial of "Troponin T, Fast Skeletal Muscle (TNNT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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