Rabbit anti-Human, Rat TAF1B Polyclonal Antibody | anti-TAF1B antibody

TAF1B Antibody

Cat. Num. AAA9603917

• Gene Names: TAF1B; SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TAF1B; Polyclonal Antibody; TAF1B Antibody; MGC:9349; RAF1B; RAFI63; SL1; TAB1 B; TAF1B TATA box binding protein (TBP) associated factor; RNA polymerase I; B; 63kDa; TAFI63; TATA box binding protein associated factor 1B; TATA box binding protein associated factor RNA polymerase I subunit B; anti-TAF1B antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TAF1B antibody detects endogenous levels of total TAF1B
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 333

• Applicable Applications for anti-TAF1B antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus.
• Conjugation: Unconjugated

Western Blot (WB)

(Western blot analysis of TAF1B expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9603917 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using TAF1B Antibody. Lane 1: mouse brain treated with blocking peptide. Lane 2: mouse brain; Lane 3: rat brain; )

Related Product Information for anti-TAF1B antibody

Function: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.
Subunit Structure: Interacts with FLNA (via N-terminus) (By similarity). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3.
Similarity: Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides (PubMed:21921198 and PubMed:21921199). Belongs to the RRN7/TAF1B family.

NCBI and Uniprot Product Information

• NCBI GI #: 974987474
• NCBI GeneID: 9014
• NCBI Accession #: NP_001305905.1
• NCBI GenBank Nucleotide #: NM_001318976.1
• UniProt Accession #: Q53T94; Q15574; Q8WVC3; B4DI42; F8WD72
• Molecular Weight: Observed: 69 kDa; Predicted: 69 kDa
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit B isoform b
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor, RNA polymerase I subunit B
• NCBI Official Symbol: TAF1B
• NCBI Official Synonym Symbols: SL1; RAF1B; RAFI63; TAFI63; MGC:9349
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit B
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF1B
• UniProt Synonym Gene Names: TAFI63; TBP-associated factor 1B

NCBI Description

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes one of the SL1-specific TAFs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.