Rabbit anti-Human Syk Polyclonal Antibody | anti-Sykb antibody

Syk, phosphorylated (Tyr323)

Cat. Num. AAA627160

• Gene Names: Sykb; Syk
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Syk; Polyclonal Antibody; phosphorylated (Tyr323); Anti -Syk; anti-Sykb antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Syk when phosphorylated at Tyr323. Species sequence homology: Mouse Syk phosphorylated at Tyr317 (79%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-Sykb antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000; Optimal dilution to be determined by the researcher.
• Immunogen: Synthetic phosphopeptide derived from a region of human Syk that contains tyrosine 323 (tyrosine 317 in the mouse sequence).
• Positive Control: Jurkat cells treated with H2O2
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Sykb antibody

Syk (72kD) is a non-receptor protein tyrosine kinase that plays a central role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. Syk is activated upon binding to immunoreceptor tyrosine-based activating motif (ITAM) and involved in coupling activated immunoreceptors to downstream signaling. Syk undergoes phosphorylation on multiple tyrosines within the linker region that separates the Cterminal catalytic domain from the N-terminal SH2 domains (tyrosines 323, 348 and 352 or 317, 342 and 346 using the mouse sequence). The phosphorylation of Syk tyrosine 323 - a Cbl binding site - mediates Cbl-induced negative regulation of Syk.

Product Categories/Family for anti-Sykb antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 818861
• NCBI GeneID: 20963
• UniProt Accession #: P48025; Q3UPF7; Q3V3Q2; Q6P1E0
• Molecular Weight: 71,376 Da
• NCBI Official Full Name: SYK
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: Sykb
• NCBI Official Synonym Symbols: Syk
• NCBI Protein Information: tyrosine-protein kinase SYK; OTTMUSP00000041832
• UniProt Protein Name: Tyrosine-protein kinase SYK
• UniProt Gene Name: Syk
• UniProt Synonym Gene Names: Sykb
• UniProt Entry Name: KSYK_MOUSE

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.2; TK group; Syk family

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; membrane; cell; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; nucleus; B cell receptor complex

Molecular Function: protein domain specific binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; receptor signaling protein tyrosine kinase activity; protein kinase binding; protein kinase activity; integrin binding; transferase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; transcription factor import into nucleus; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; serotonin secretion; activation of JNK activity; leukocyte adhesion; B cell receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; angiogenesis; positive regulation of gamma-delta T cell differentiation; integrin-mediated signaling pathway; neutrophil chemotaxis; adaptive immune response; regulation of immune response; cell migration; regulation of superoxide release; immune system process; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; neutrophil activation during immune response; positive regulation of peptidyl-tyrosine phosphorylation; serotonin secretion by platelet; leukocyte activation during immune response; macrophage activation during immune response; defense response to bacterium; innate immune response; positive regulation of B cell differentiation; blood vessel morphogenesis; positive regulation of alpha-beta T cell proliferation; lymph vessel development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion