Rabbit anti-Human, Rat SUV39H2 Polyclonal Antibody | anti-SUV39H2 antibody

SUV39H2, CT (Histone-lysine N-methyltransferase SUV39H2, Suppressor of Variegation 3-9 Homolog 2, Su(var)3-9 Homolog 2, Histone H3-K9 Methyltransferase 2, H3-K9-HMTase 2, Lysine N-methyltransferase 1B, KMT1B) (APC)

Cat. Num. AAA6501725

• Gene Names: SUV39H2; KMT1B
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: SUV39H2; Polyclonal Antibody; CT (Histone-lysine N-methyltransferase SUV39H2; Suppressor of Variegation 3-9 Homolog 2; Su(var)3-9 Homolog 2; Histone H3-K9 Methyltransferase 2; H3-K9-HMTase 2; Lysine N-methyltransferase 1B; KMT1B) (APC); anti-SUV39H2 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SUV39H2. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SUV39H2 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 360-390 in the C-terminal region of human SUV39H2, UniProt Accession #Q9H5I1. Species sequence homology: monkey
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 and HL60 cell line lysates (35ug/lane) usingMBS626595. SUV39H2 (arrow) was detected usingMBS626595.)

Western Blot (WB)

(Western Blot analysis of lysates from A431, K562, CEM cell line and rat brain tissue lyaste (from left to right, 35ug/lane), using MBS626595 (1:1000) at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10,000 was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS626595, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS626595 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-SUV39H2 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 13375930
• NCBI GeneID: 79723
• NCBI Accession #: NP_078946
• NCBI GenBank Nucleotide #: NM_001193424.1
• UniProt Accession #: Q9H5I1
• Molecular Weight: 38 kDa
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV39H2 isoform 2
• NCBI Official Synonym Full Names: suppressor of variegation 3-9 homolog 2
• NCBI Official Symbol: SUV39H2
• NCBI Official Synonym Symbols: KMT1B
• NCBI Protein Information: histone-lysine N-methyltransferase SUV39H2
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV39H2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SUV39H2
• UniProt Synonym Gene Names: KMT1B; H3-K9-HMTase 2
• UniProt Entry Name: SUV92_HUMAN

Uniprot Description

SUV39H2: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: nucleoplasm; nuclear heterochromatin; chromatin; chromosome, pericentric region

Molecular Function: protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K9 specific); chromatin binding

Biological Process: chromatin assembly or disassembly; chromatin remodeling; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of transcription, DNA-dependent; male meiosis