Rat Thrombopoietin ELISA Kit | TPO elisa kit

Rat Thrombopoietin ELISA Kit

Cat. Num. AAA764453

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Reactivity: Rat
• Synonyms: Thrombopoietin; Rat Thrombopoietin ELISA Kit; TPO (Thrombopoietin)/MGDF/MK-CSF/THPO; TPO elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNX. No significant cross-reactivity or interference between TNX and analogues was observed.
• Sequence Length: 353

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/ml
• Sensitivity: < 0.938ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNX were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNX were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPO elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TNX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TNX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNX can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 586798208
• NCBI GeneID: 7066
• NCBI Accession #: NP_001276927.1
• NCBI GenBank Nucleotide #: NM_001289998.1
• Molecular Weight: 33,604 Da
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF; ML; MGDF
• UniProt Entry Name: TPO_HUMAN

NCBI Description

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; hormone activity

Biological Process: blood coagulation; cell proliferation; multicellular organismal development; myeloid cell differentiation; platelet activation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade

Disease: Thrombocythemia 1