Immunohistochemistry (IHC)
(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse ACTA2 / Smooth Muscle Actin Monoclonal Antibody | anti-ACTA2 antibody
Anti-ACTA2 / Smooth Muscle Actin Antibody (clone 1H8) IHC-plus
Flow Cytometry (1:200 - 1:400)
ICC (1:200 - 1:1000)
IF (1:1000)
IHC-P (2.5 - 5 ug/ml)
WB (1:500 - 1:2000)
Short term: 4 degree C
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescence of NIH/3T3 cells using ACTA2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Immunofluorescence (IF)
(Immunofluorescence of NIH/3T3 cells using ACTA2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Western Blot (WB)
(Western blot using ACTA2 mouse monoclonal antibody against HeLa (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.)
Western Blot (WB)
(Western blot using ACTA2 mouse monoclonal antibody against HeLa (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.)
ELISA (EIA)
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
ELISA (EIA)
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Uniprot Description
ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
Protein type: Cytoskeletal; Contractile; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q23.3
Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; filopodium; lamellipodium; protein complex; smooth muscle contractile fiber
Molecular Function: ATP binding; protein kinase binding
Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction
Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome
Research Articles on ACTA2
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Product Notes
The ACTA2 acta2 (Catalog #AAA249454) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ACTA2 / Smooth Muscle Actin Antibody (clone 1H8) IHC-plus reacts with Mouse, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ACTA2 / Smooth Muscle Actin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000) Flow Cytometry (1:200 - 1:400) ICC (1:200 - 1:1000) IF (1:1000) IHC-P (2.5 - 5 ug/ml) WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTA2 / Smooth Muscle Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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