Western Blot (WB)
(Anti-UQCRC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HEK-293 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:45 kDa)
Rabbit anti-Human UQCRC1 Polyclonal Antibody | anti-UQCRC1 antibody
Anti-UQCRC1 Antibody, Rabbit Polyclonal
Gene Names
UQCRC1; QCR1; UQCR1; D3S3191
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
UQCRC1; Polyclonal Antibody; Anti-UQCRC1 Antibody; Rabbit Polyclonal; UQCRC1 Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquinol-cytochrome c reductase core protein I; Anti-D3S3191 Antibody; Anti-QCR1 Antibody; Anti-UQCR1 Antibody; anti-UQCRC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human UQCRC1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-UQCRC1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Human UQCRC1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human UQCRC1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-UQCRC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HEK-293 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:45 kDa)
Western Blot (WB)
(Anti-UQCRC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HEK-293 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:45 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human UQCRC1 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human UQCRC1 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human UQCRC1 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human UQCRC1 in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-UQCRC1 antibody
Ubiquinol cytochrome c reductase core protein I (UQCRC1) is a component of the complex III in the respiratory chain and is expressed abundantly in neurons and astrocytes. UQCRC1 may also be important by forming complex III to maintain normal brain ischemic tolerance, learning, and memory. UQCRC1 is a nuclear-encoded protein localized to the inner mitochondrial membrane and plays a key role in influencing mitochondrial function. Increasing evidence supports that UQCRC1 overexpression takes part in cardioprotection. UQCRC1 overexpression protects H9c2 cardiac cells against mimic ischemia/reperfusion injury through mediating PI3K/Akt/GSK-3beta pathway to regulate apoptosis-related proteins. UQCRC1 was downregulated in human colorectal cancer (CRC). Downregulation of UQCRC1 was correlated with increased lymph node metastasis and finally associated with decreased survival in CRC.
References
Yi T, et al. (2020) Ubiquinol-cytochrome c reductase core protein 1 overexpression protects h9c2 cardiac cells against mimic ischemia/reperfusion injury through pi3k/akt/gsk-3beta pathway. Biochem Biophys Res Commun 529 (4): 904-909.Shan W, et al. (2019) Critical role of uqcrc1 in embryo survival, brain ischemic tolerance and normal cognition in mice. Cell Mol Life Sci 76 (7): 1381-1396.Li W, et al. (2019) Uqcrc1 downregulation is correlated with lymph node metastasis and poor prognosis in crc. Eur J Surg Oncol 45 (6): 1005-1010.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
480
NCBI Official Full Name
Cytochrome b-c1 complex subunit 1, mitochondrial
NCBI Official Synonym Full Names
ubiquinol-cytochrome c reductase core protein I
NCBI Official Symbol
UQCRC1
NCBI Official Synonym Symbols
QCR1; UQCR1; D3S3191
NCBI Protein Information
cytochrome b-c1 complex subunit 1, mitochondrial; core protein I; complex III subunit 1; ubiquinol-cytochrome-c reductase complex core protein 1
UniProt Protein Name
Cytochrome b-c1 complex subunit 1, mitochondrial
UniProt Gene Name
UQCRC1
UniProt Entry Name
QCR1_HUMAN
Uniprot Description
UQCRC1: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1. Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily.
Protein type: EC 1.10.2.2; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex III; mitochondrial respiratory chain
Molecular Function: metal ion binding; ubiquinol-cytochrome-c reductase activity; protein complex binding
Biological Process: response to alkaloid; cellular metabolic process; aerobic respiration; response to activity; oxidative phosphorylation; mitochondrial electron transport, ubiquinol to cytochrome c
Research Articles on UQCRC1
Similar Products
Product Notes
The UQCRC1 uqcrc1 (Catalog #AAA8103056) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UQCRC1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UQCRC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the UQCRC1 uqcrc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UQCRC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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