Western Blot (WB)
(GADD45G Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GADD45G antibody detected the GADD45G protein (arrow).)
Rabbit anti-Human GADD45G Polyclonal Antibody | anti-GADD45G antibody
GADD45G, NT (GADD45G, CR6, DDIT2, Growth arrest and DNA damage-inducible protein GADD45 gamma, Cytokine-responsive protein CR6, DNA damage-inducible transcript 2 protein) (PE)
Gene Names
GADD45G; CR6; DDIT2; GRP17; GADD45gamma
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
GADD45G; Polyclonal Antibody; NT (GADD45G; CR6; DDIT2; Growth arrest and DNA damage-inducible protein GADD45 gamma; Cytokine-responsive protein CR6; DNA damage-inducible transcript 2 protein) (PE); DNA damage-inducible transcript 2 protein; anti-GADD45G antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-GADD45G antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
GADD45G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GADD45G.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(GADD45G Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GADD45G antibody detected the GADD45G protein (arrow).)
Western Blot (WB)
(GADD45G Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GADD45G antibody detected the GADD45G protein (arrow).)
Related Product Information for anti-GADD45G antibody
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.
Product Categories/Family for anti-GADD45G antibody
References
Flores, O., et al. Endocrinology 151(10):4654-4664(2010). Zhang, W., et al. J. Cancer Res. Clin. Oncol. 136(8):1267-1273(2010). Zhu, N., et al. Mol. Biol. Rep. 36(8):2075-2085(2009). Yu, S., et al. Am. J. Nephrol. 30(2):135-139(2009). Palmieri, R.T., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3567-3572(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,121 Da
NCBI Official Full Name
growth arrest and DNA damage-inducible protein GADD45 gamma
NCBI Official Synonym Full Names
growth arrest and DNA-damage-inducible, gamma
NCBI Official Symbol
GADD45G
NCBI Official Synonym Symbols
CR6; DDIT2; GRP17; GADD45gamma
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 gamma; DDIT-2; GADD45-gamma; gadd-related protein, 17 kD; cytokine-responsive protein CR6; DNA damage-inducible transcript 2 protein
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 gamma
Protein Family
UniProt Gene Name
GADD45G
UniProt Synonym Gene Names
CR6; DDIT2; DDIT-2
UniProt Entry Name
GA45G_HUMAN
NCBI Description
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]
Uniprot Description
GADD45G: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. Belongs to the GADD45 family.
Protein type: Apoptosis; DNA repair, damage; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 9q22.1-q22.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: activation of MAPKK activity; apoptosis; regulation of cell cycle; positive regulation of apoptosis; multicellular organismal development; activation of MAPKKK activity; positive regulation of JNK cascade; response to stress; negative regulation of protein kinase activity; cell differentiation
Research Articles on GADD45G
Similar Products
Product Notes
The GADD45G gadd45g (Catalog #AAA6300971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GADD45G, NT (GADD45G, CR6, DDIT2, Growth arrest and DNA damage-inducible protein GADD45 gamma, Cytokine-responsive protein CR6, DNA damage-inducible transcript 2 protein) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GADD45G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GADD45G gadd45g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GADD45G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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