Rabbit anti-Mouse STAT3 Polyclonal Antibody | anti-STAT3 antibody

RABBIT ANTI STAT3

Cat. Num. AAA224842

• Gene Names: Stat3; Aprf; AW109958; 1110034C02Rik
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: STAT3; Polyclonal Antibody; RABBIT ANTI STAT3; anti-STAT3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: STAT3
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 770

• Applicable Applications for anti-STAT3 antibody: Western Blot (WB)
• Application Notes: Western Blot: 0.5; 4.0 ug/ml
• Reactivity Notes: Human, Rat
• Preservative Stabilisers: 30% Glycerol, 0.5% Bovine Serum Albumin, 0.01% Thiomersal
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at -20 degree C Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of Jurkat cell lysate (lane 1 & 2), 3T3 cell lysate (lane 3) and rat kidney tissue lysate (lane 4) probed with Rabbit anti STAT3 antibody)

Related Product Information for anti-STAT3 antibody

Rabbit anti STAT3 antibody recognizes signal transducer and activator of transcription 3 (STAT3), a member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus. STAT3 is phosphorylated my members of the Janus kinase (JAK) family in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. Phosphorylation results in STAT activation and the formation of STAT3 homo- or STAT3/STAT1 and STAT3/STAT5 heterodimers. STAT dimers translocate from the cytoplasm to the nucleus where they act as transcription factors. Deregulated JAK/STAT signaling has been implicated in a variety of cancers; STATs can regulate the tumor microenvironment and trigger a shift from cancer inhibition to promotion. STAT3 is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Consistent STAT3 activation has been demonstrated to play a role in cell proliferation and tumor promoting inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 1711553
• NCBI GeneID: 20848
• UniProt Accession #: P42227; A2A5D1; B7ZC17
• Molecular Weight: 87,967 Da
• NCBI Official Full Name: Signal transducer and activator of transcription 3
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: Stat3
• NCBI Official Synonym Symbols: Aprf; AW109958; 1110034C02Rik
• NCBI Protein Information: signal transducer and activator of transcription 3
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat3
• UniProt Synonym Gene Names: Aprf
• UniProt Entry Name: STAT3_MOUSE

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Transcription factor; DNA-binding; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nuclear chromatin; nucleoplasm; nucleus; plasma membrane

Molecular Function: CCR5 chemokine receptor binding; chromatin DNA binding; DNA binding; glucocorticoid receptor binding; identical protein binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; protein kinase binding; protein phosphatase binding; sequence-specific DNA binding; signal transducer activity; transcription factor activity; transcription factor binding

Biological Process: acute-phase response; astrocyte differentiation; cell proliferation; cellular response to hormone stimulus; cytokine and chemokine mediated signaling pathway; eating behavior; eye photoreceptor cell differentiation; glucose homeostasis; intracellular receptor-mediated signaling pathway; JAK-STAT cascade; leptin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of glycolysis; phosphorylation; positive regulation of cell proliferation; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus; radial glial cell differentiation; regulation of cell cycle; regulation of mitochondrial membrane permeability; regulation of multicellular organism growth; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to estradiol stimulus; sexual reproduction; signal transduction; stem cell maintenance; thermoregulation; transcription from RNA polymerase II promoter; transcription, DNA-dependent