Rabbit anti-Human STAT3 Polyclonal Antibody | anti-Stat3 antibody

STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)

Cat. Num. AAA628931

• Gene Names: Stat3; Aprf; AW109958; 1110034C02Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STAT3; Polyclonal Antibody; phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3; Acute-phase Response Factor; APRF; FLJ20882; MGC16063); Anti -STAT3; anti-Stat3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT3 when phosphorylated at Tyr705.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Stat3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr705 of human STAT3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS628931 in mouse liver tissue lysate (35ug/lane). Phospho-STAT3(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS628931, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma)

Related Product Information for anti-Stat3 antibody

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.

Product Categories/Family for anti-Stat3 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 458706
• NCBI GeneID: 20848
• UniProt Accession #: P42227; A2A5D1; B7ZC17; Q3U5Q4; Q3U6S9; Q3ULI4; Q6GU23; Q8CFJ6; Q99ML3
• Molecular Weight: 88,054 Da
• NCBI Official Full Name: Stat3
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: Stat3
• NCBI Official Synonym Symbols: Aprf; AW109958; 1110034C02Rik
• NCBI Protein Information: signal transducer and activator of transcription 3; OTTMUSP00000002052; OTTMUSP00000002053; OTTMUSP00000045315; OTTMUSP00000045316; acute phase response factor; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat3
• UniProt Synonym Gene Names: Aprf
• UniProt Entry Name: STAT3_MOUSE

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Nuclear receptor co-regulator; Transcription factor

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; plasma membrane; intracellular; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; protein binding; ligand-dependent nuclear receptor activity; DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor activity; transcription factor binding; protein phosphatase binding; glucocorticoid receptor binding; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; eating behavior; cytokine and chemokine mediated signaling pathway; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; cell proliferation; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation