Chicken Signal Transducer And Activator Of Transcription 3 ELISA Kit | STAT3 elisa kit

Chicken Signal Transducer And Activator Of Transcription 3 ELISA Kit

Cat. Num. AAA734510

• Gene Names: Stat3; Aprf; AW109958; 1110034C02Rik
• Reactivity: Chicken
• Synonyms: Signal Transducer And Activator Of Transcription 3; Chicken Signal Transducer And Activator Of Transcription 3 ELISA Kit; STAT3 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL
• Intended Uses: Thisx STAT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken STAT3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT3 elisa kit

Principle of the Assay: STAT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STAT3 antibody and an STAT3-HRP conjugate. The assay sample and buffer are incubated together with STAT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAT3 concentration since STAT3 from samples and STAT3-HRP conjugate compete for the anti-STAT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by STAT3 from the sample, fewer sites are left to bind STAT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for STAT3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 458706
• NCBI GeneID: 20848
• UniProt Accession #: P42227; A2A5D1; B7ZC17; Q3U5Q4; Q3U6S9; Q3ULI4; Q6GU23; Q8CFJ6; Q99ML3
• Molecular Weight: 88,054 Da
• NCBI Official Full Name: Stat3
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: Stat3
• NCBI Official Synonym Symbols: Aprf; AW109958; 1110034C02Rik
• NCBI Protein Information: signal transducer and activator of transcription 3; acute phase response factor; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat3
• UniProt Synonym Gene Names: Aprf
• UniProt Entry Name: STAT3_MOUSE

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; cytoplasm; plasma membrane; intracellular; nucleus

Molecular Function: protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; signal transducer activity; DNA binding; sequence-specific DNA binding; transcription factor binding; protein phosphatase binding; transcription factor activity; protein kinase binding; glucocorticoid receptor binding; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; eating behavior; cytokine and chemokine mediated signaling pathway; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; cell proliferation; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation