Canine Signal Transducer And Activator Of Transcription 3 ELISA Kit | STAT3 elisa kit

Canine Signal Transducer And Activator Of Transcription 3 ELISA Kit

Cat. Num. AAA735481

• Gene Names: Stat3; Aprf; AW109958; 1110034C02Rik
• Reactivity: Canine
• Synonyms: Signal Transducer And Activator Of Transcription 3; Canine Signal Transducer And Activator Of Transcription 3 ELISA Kit; STAT3 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of STAT3. No significant cross-reactivity or interference between STAT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STAT3 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT3 elisa kit

Intended Uses: This STAT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine STAT3. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: STAT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-STAT3 antibody and an STAT3-HRP conjugate. The assay sample and buffer are incubated together with STAT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STAT3 concentration since STAT3 from samples and STAT3-HRP conjugate compete for the anti-STAT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by STAT3 from the sample, fewer sites are left to bind STAT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for STAT3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 458706
• NCBI GeneID: 20848
• Molecular Weight: 87,967 Da
• NCBI Official Full Name: Stat3
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: Stat3
• NCBI Official Synonym Symbols: Aprf; AW109958; 1110034C02Rik
• NCBI Protein Information: signal transducer and activator of transcription 3; acute phase response factor; acute-phase response factor
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat3
• UniProt Synonym Gene Names: Aprf
• UniProt Entry Name: STAT3_MOUSE

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; plasma membrane; intracellular; nucleus

Molecular Function: protein dimerization activity; protein binding; signal transducer activity; ligand-dependent nuclear receptor activity; DNA binding; sequence-specific DNA binding; protein phosphatase binding; transcription factor activity; transcription factor binding; protein kinase binding; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; signal transduction; glucose homeostasis; response to estradiol stimulus; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; eating behavior; cytokine and chemokine mediated signaling pathway; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; cell proliferation; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation