Rabbit anti-Human STARD5 Polyclonal Antibody | anti-STARD5 antibody

STARD5, NT (STARD5, StAR-related lipid transfer protein 5, START domain-containing protein 5) (FITC)

Cat. Num. AAA6351777

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: STARD5; Polyclonal Antibody; NT (STARD5; StAR-related lipid transfer protein 5; START domain-containing protein 5) (FITC); START domain-containing protein 5; anti-STARD5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-STARD5 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: STARD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-63 amino acids from the N-terminal region of human STARD5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(STARD5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the STARD5 antibody detected the STARD5 protein (arrow).)

Immunohistochemistry (IHC)

(STARD5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of STARD5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(STARD5 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-STARD5 antibody

Cholesterol homeostasis is regulated, at least in part, by sterol regulatory element (SRE)-binding proteins (e.g., SREBP1; MIM 184756) and by liver X receptors (e.g., LXRA; MIM 602423). Upon sterol depletion, LXRs are inactive and SREBPs are cleaved, after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers, such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200-to 210-amino acid STAR-related lipid transfer (START) domain, including STARD5 (Soccio et al., 2002 [PubMed 12011452]).

Product Categories/Family for anti-STARD5 antibody

Antibodies; Lipoproteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Chen, Y.C., et al. Am. J. Physiol. Renal Physiol. 297 (2), F380-F388 (2009): Rodriguez-Agudo, D., et al. J. Lipid Res. 47(6):1168-1175(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 32526915
• NCBI GeneID: 80765
• NCBI Accession #: NP_871629.1
• NCBI GenBank Nucleotide #: NM_181900.2
• UniProt Accession #: Q9NSY2; P59094
• Molecular Weight: 7,094 Da
• NCBI Official Full Name: stAR-related lipid transfer protein 5
• NCBI Official Synonym Full Names: StAR-related lipid transfer (START) domain containing 5
• NCBI Official Symbol: STARD5
• NCBI Protein Information: stAR-related lipid transfer protein 5; START domain containing 5; START domain-containing protein 5
• UniProt Protein Name: StAR-related lipid transfer protein 5
• Protein Family: StAR-related lipid transfer protein
• UniProt Gene Name: STARD5
• UniProt Synonym Gene Names: StARD5
• UniProt Entry Name: STAR5_HUMAN

NCBI Description

Cholesterol homeostasis is regulated, at least in part, by sterol regulatory element (SRE)-binding proteins (e.g., SREBP1; MIM 184756) and by liver X receptors (e.g., LXRA; MIM 602423). Upon sterol depletion, LXRs are inactive and SREBPs are cleaved, after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers, such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain, including STARD5 (Soccio et al., 2002 [PubMed 12011452]).[supplied by OMIM, Mar 2008]

Uniprot Description

STARD5: May be involved in the intracellular transport of sterols or other lipids. May bind cholesterol or other sterols. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: cytosol

Molecular Function: bile acid binding

Biological Process: steroid metabolic process; C21-steroid hormone biosynthetic process; lipid transport