SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
PTGR1 recombinant protein
PTGR1, 1-329aa, Human, His tag, E Coli,
Gene Names
PTGR1; PGR1; ZADH3; LTB4DH
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
PTGR1; 1-329aa; Human; His tag; E Coli; ; prostaglandin reductase 1; LTB4DH; PGR1; ZADH3; PTGR1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH7.5, 10% glycerol, 1mM DTT, 200mM NaCl
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMVRTK TWTLKKHFVG YPTNSDFELK TAELPPLKNG EVLLEALFLT VDPYMRVAAK RLKEGDTMMG QQVAKVVESK NVALPKGTIV LASPGWTTHS ISDGKDLEKL LTEWPDTIPL SLALGTVGMP GLTAYFGLLE ICGVKGGETV MVNAAAGAVG SVVGQIAKLK GCKVVGAVGS DEKVAYLQKL GFDVVFNYKT VESLEETLKK ASPDGYDCYF DNVGGEFSNT VIGQMKKFGR IAICGAISTY NRTGPLPPGP PPEIVIYQEL RMEAFVVYRW QGDARQKALK DLLKWVLEGK IQYKEYIIEG FENMPAAFMG MLKGDNLGKT IVKA
Sequence Length
329
Applicable Applications for PTGR1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Domain
1-329aa
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage
aliquot and store at -20C to -80C.
Avoid repeated freezing and thawing cycles.
aliquot and store at -20C to -80C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for PTGR1 recombinant protein
PTGR1, also known as LTB4DH, belongs to the NADP-dependent oxidoreductase L4BD family. This enzyme catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4. It is highly expressed in the kidney, liver, and intestine but not in leukocytes. Recombinant human PTGR1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PTGR1 recombinant protein
References
Yokomizo T., et al. (1996) J. Biol. Chem. 271:2844-2850; Tai, H.H., et al. (2002). Prostaglandins Other Lipid Mediat. 68-69: 483-493.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38.6kDa (354aa), confirmed by MALDI-TOF
NCBI Official Full Name
prostaglandin reductase 1 isoform 1
NCBI Official Synonym Full Names
prostaglandin reductase 1
NCBI Official Symbol
PTGR1
NCBI Official Synonym Symbols
PGR1; ZADH3; LTB4DH
NCBI Protein Information
prostaglandin reductase 1
UniProt Protein Name
Prostaglandin reductase 1
Protein Family
UniProt Gene Name
PTGR1
UniProt Synonym Gene Names
LTB4DH; PRG-1
NCBI Description
This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]
Uniprot Description
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha (). Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.
Research Articles on PTGR1
Similar Products
Product Notes
The PTGR1 ptgr1 (Catalog #AAA204591) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag!!Domain||1-329aa. AAA Biotech's PTGR1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PTGR1 ptgr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSEFMVRTK TWTLKKHFVG YPTNSDFELK TAELPPLKNG EVLLEALFLT VDPYMRVAAK RLKEGDTMMG QQVAKVVESK NVALPKGTIV LASPGWTTHS ISDGKDLEKL LTEWPDTIPL SLALGTVGMP GLTAYFGLLE ICGVKGGETV MVNAAAGAVG SVVGQIAKLK GCKVVGAVGS DEKVAYLQKL GFDVVFNYKT VESLEETLKK ASPDGYDCYF DNVGGEFSNT VIGQMKKFGR IAICGAISTY NRTGPLPPGP PPEIVIYQEL RMEAFVVYRW QGDARQKALK DLLKWVLEGK IQYKEYIIEG FENMPAAFMG MLKGDNLGKT IVKA. It is sometimes possible for the material contained within the vial of "PTGR1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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