Western Blot (WB)
(Western Blot Extracts prepared from CEF cells transfected with Src (1) or left untransfected (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for two hours at room temperature and incubated with a 1:1000 dilution of Src pan antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method)
Rabbit anti-Human, Mouse Src, pan Polyclonal Antibody
Src, pan (pp60Src, pp60c-Src)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Src; pan; Polyclonal Antibody; pan (pp60Src; pp60c-Src); Anti -Src; anti-Src; pan antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes human and mouse Src. Species sequence homology: Rat (100%) and chicken Src (93%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-Src, pan antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Dilution: Western Blot: 1:1000
Immunogen
Synthetic peptide corresponding to aa6-19 from human Src.
Positive Control
CEF cell extracts +/- Src; human platelets
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot Extracts prepared from CEF cells transfected with Src (1) or left untransfected (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for two hours at room temperature and incubated with a 1:1000 dilution of Src pan antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method)
Western Blot (WB)
(Western Blot Extracts prepared from CEF cells transfected with Src (1) or left untransfected (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for two hours at room temperature and incubated with a 1:1000 dilution of Src pan antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method)
Related Product Information for anti-Src, pan antibody
Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets. Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins. Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor. In addition, Src is thought to play a role in physiological/pathophysiological processes in the central nervous system. This antibody is useful to determine total levels of Src protein.
Product Categories/Family for anti-Src, pan antibody
Similar Products
Product Notes
The Src, pan (Catalog #AAA614852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Src, pan (pp60Src, pp60c-Src) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Src, pan can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Src, pan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src, pan, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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