Rabbit Src Polyclonal Antibody | anti-Src antibody

Src, phosphorylated (Tyr418) (Proto-oncogene Tyrosine-protein Kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src, SRC1)

Cat. Num. AAA629355

• Gene Names: Src; AW259666; pp60c-src
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Src; Polyclonal Antibody; phosphorylated (Tyr418) (Proto-oncogene Tyrosine-protein Kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC1); Anti -Src; anti-Src antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Scr when phosphorylated at Tyr418. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Src antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot:1:500-1:1,000; Immunohistochemistry:1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human Scr around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Src antibody

Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic SH2 domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This activity is negatively regulated when tyrosine 529 of Src is phosphorylated by Csk. This conformation holds Src in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus.

Product Categories/Family for anti-Src antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 62635494
• NCBI GeneID: 20779
• UniProt Accession #: P05480; Q2M4I4
• Molecular Weight: 60,645 Da
• NCBI Official Full Name: Src
• NCBI Official Synonym Full Names: Rous sarcoma oncogene
• NCBI Official Symbol: Src
• NCBI Official Synonym Symbols: AW259666; pp60c-src
• NCBI Protein Information: neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src
• UniProt Protein Name: Neuronal proto-oncogene tyrosine-protein kinase Src
• Protein Family: Src-like-adapter
• UniProt Gene Name: Src
• UniProt Synonym Gene Names: p60-Src
• UniProt Entry Name: SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.2; Oncoprotein; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; mitochondrion; membrane; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; plasma membrane; actin filament; intracellular; caveola; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; hormone receptor binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; oogenesis; regulation of estrogen receptor signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; progesterone receptor signaling pathway; immune system process; regulation of cell cycle; platelet-derived growth factor receptor signaling pathway; uterus development; cell cycle; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption