Rat Thyroid Peroxidase (TPO) ELISA Kit | TPO elisa kit

Rat Thyroid Peroxidase (TPO) ELISA Kit

Cat. Num. AAA2702018

• Gene Names: TPO; MSA; TPX; TDH2A
• Reactivity: Rat
• Synonyms: Thyroid Peroxidase (TPO); Rat Thyroid Peroxidase (TPO) ELISA Kit; TPX; thyroperoxidase; TPO elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroid Peroxidase (TPO).; No significant cross-reactivity or interference between Thyroid Peroxidase (TPO) and analogues was observed.
• Sequence Length: 933

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.128ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Peroxidase (TPO) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Peroxidase (TPO) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPO elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Peroxidase (TPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Peroxidase (TPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Peroxidase (TPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Peroxidase (TPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 332078490
• NCBI GeneID: 7173
• NCBI Accession #: NP_001193673.1
• NCBI GenBank Nucleotide #: NM_001206744.1
• UniProt Accession #: P07202; P09934; P09935; Q8IUL0; Q8NF94; Q8NF95; Q8NF96; Q8NF97; Q8TCI9
• Molecular Weight: 92,063 Da
• NCBI Official Full Name: thyroid peroxidase isoform a
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: TPO
• NCBI Official Synonym Symbols: MSA; TPX; TDH2A
• NCBI Protein Information: thyroid peroxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: TPO
• UniProt Synonym Gene Names: TPO

NCBI Description

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

Uniprot Description

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.