Typical Testing Data/Standard Curve (for reference only)
Rat Thyroid Peroxidase (TPO) ELISA Kit | TPO elisa kit
Rat Thyroid Peroxidase (TPO) ELISA Kit
Gene Names
TPO; MSA; TPX; TDH2A
Reactivity
Rat
Synonyms
Thyroid Peroxidase (TPO); Rat Thyroid Peroxidase (TPO) ELISA Kit; TPX; thyroperoxidase; TPO elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Thyroid Peroxidase (TPO).
No significant cross-reactivity or interference between Thyroid Peroxidase (TPO) and analogues was observed.
No significant cross-reactivity or interference between Thyroid Peroxidase (TPO) and analogues was observed.
Sequence Length
933
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.128ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Thyroid Peroxidase (TPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Thyroid Peroxidase (TPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TPO elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Peroxidase (TPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Peroxidase (TPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Peroxidase (TPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Peroxidase (TPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,063 Da
NCBI Official Full Name
thyroid peroxidase isoform a
NCBI Official Synonym Full Names
thyroid peroxidase
NCBI Official Symbol
TPO
NCBI Official Synonym Symbols
MSA; TPX; TDH2A
NCBI Protein Information
thyroid peroxidase
UniProt Protein Name
Thyroid peroxidase
Protein Family
UniProt Gene Name
TPO
UniProt Synonym Gene Names
TPO
NCBI Description
This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
Uniprot Description
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.
Research Articles on TPO
Similar Products
Product Notes
The Rat TPO tpo (Catalog #AAA2702018) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2702018 ELISA Kit recognizes Rat TPO. It is sometimes possible for the material contained within the vial of "Thyroid Peroxidase (TPO), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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