Goat SPOCK2 Polyclonal Antibody
SPOCK2 (Testican 2)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SPOCK2; Polyclonal Antibody; SPOCK2 (Testican 2); Anti -SPOCK2 (Testican 2); anti-SPOCK2 antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SPOCK2. Species crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-SPOCK2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation.
Dilution: Direct ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Dilution: Direct ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunogen
Recombinant corresponding to aa23-424 from human SPOCK 2 expressed in NSO cells.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SPOCK2 antibody
Testican 2 (also SPOCK2) is a 60-140kD, secreted multidomain proteoglycan that belongs to the BM-40/SPARC family of extracellular proteins. It is expressed by neurons (both excitatory and GABAergic) plus brain endothelial cells, and appears to abrogate testican 1 and 3 mediated inhibition of MT1- and MT3-MMP activity. Mature human Testican 2 is 402aa in length (aa23- 424). It contains a unique 66aa N-terminus, followed by a Kazal-like follistatin domain (aa89- 192), a collagen-binding domain (aa210-219), a Ca++ binding region, one thyroglobulin-like segment (aa310-376) and an acidic C-terminus that contains two potential GAG attachment sites at Ser383 and Ser388. The wide variation in native MW reflects the capabilities of differing cells to add glycan adducts to the molecule. Typically, it appears to lie belween 80-125kD. There are potential splice variants. One shows an alternative start site at Met110, a second shows a deletion of (aa64-66), while a third contains a 14aa substitution for (aa64-242). Mature human Testican 2 is 94% aa identical to mouse and rat Testican 2.
Product Categories/Family for anti-SPOCK2 antibody
NCBI and Uniprot Product Information
Uniprot Description
SPOCK2: May participate in diverse steps of neurogenesis. Binds calcium.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10pter-q25.3
Cellular Component: proteinaceous extracellular matrix
Molecular Function: glycosaminoglycan binding; extracellular matrix binding; calcium ion binding
Biological Process: extracellular matrix organization and biogenesis; synaptogenesis; regulation of cell differentiation; signal transduction; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan
Similar Products
Product Notes
The SPOCK2 (Catalog #AAA6011974) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SPOCK2 (Testican 2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPOCK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot, Immunoprecipitation. Dilution: Direct ELISA: 0.5-1ug/ml Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the SPOCK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPOCK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
