Western Blot (WB)
(Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.)
Rabbit anti-Human SPINSTER Polyclonal Antibody | anti-SPNS1 antibody
SPINSTER Antibody
Gene Names
SPNS1; LAT; nrs; SPIN1; SPINL; HSpin1; PP2030
Reactivity
Human
Applications
ELISA, Western Blot
Purity
SPINSTER antibody is affinity chromatography purified via peptide column.
Synonyms
SPINSTER; Polyclonal Antibody; SPINSTER Antibody; LAT; nrs; SPIN1; SPINL; HSpin1; PP2030; PP20300; Protein spinster homolog 1; spinster homolog 1 (Drosophila); anti-SPNS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.
Purity/Purification
SPINSTER antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
454
Applicable Applications for anti-SPNS1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 mug/ml.
Conjugate
Unconjugated
Immunogen
SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER.
Buffer
SPINSTER antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SPINSTER antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.)
Western Blot (WB)
(Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.)
Related Product Information for anti-SPNS1 antibody
SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 58 kDa
Observed: 70 kDa
Observed: 70 kDa
NCBI Official Full Name
protein spinster homolog 1 isoform 4
NCBI Official Synonym Full Names
spinster homolog 1 (Drosophila)
NCBI Official Symbol
SPNS1
NCBI Official Synonym Symbols
LAT; nrs; SPIN1; SPINL; HSpin1; PP2030
NCBI Protein Information
protein spinster homolog 1; spinster-like protein 1
UniProt Protein Name
Protein spinster homolog 1
Protein Family
UniProt Gene Name
SPNS1
UniProt Synonym Gene Names
SPIN1
UniProt Entry Name
SPNS1_HUMAN
Uniprot Description
SPNS1: Sphingolipid transporter. May be involved in necrotic or autophagic cell death. Belongs to the major facilitator (TC 2.A.1) superfamily. Spinster (TC 2.A.1.49) family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: lysosomal membrane; mitochondrial inner membrane; integral to membrane; vesicle
Molecular Function: protein binding; sphingolipid transporter activity
Biological Process: lipid transport; locomotion; transmembrane transport
Research Articles on SPNS1
Similar Products
Product Notes
The SPNS1 spns1 (Catalog #AAA153414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPINSTER Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPINSTER can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 mug/ml. Researchers should empirically determine the suitability of the SPNS1 spns1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPINSTER, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
