Western Blot (WB)
((0. 03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Radixin Polyclonal Antibody | anti-RDX antibody
Goat anti-Radixin Antibody
Western Blot: Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem.1995 Oct 27;270(43): 25324-7. PMID: 7592691). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of NIH3T3 (A), HeLa (B), Jurkat (C), and A431 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of NIH3T3 (A), HeLa (B), Jurkat (C), and A431 (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Uniprot Description
Radixin: a cytoskeletal protein of the ERM family which includes ezrin and moiesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. May play a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: apical plasma membrane; cleavage furrow; cortical actin cytoskeleton; extracellular space; extrinsic to membrane; filopodium; focal adhesion; lamellipodium; microvillus; myelin sheath; plasma membrane; ruffle; stereocilium
Molecular Function: actin binding; ATPase binding; protein binding; protein domain specific binding; protein homodimerization activity; protein kinase A binding
Biological Process: actin filament capping; apical protein localization; establishment of protein localization; microvillus biogenesis; negative regulation of cell size; negative regulation of homotypic cell-cell adhesion; positive regulation of cell migration; regulation of actin filament bundle formation; regulation of cell shape; regulation of cell size; regulation of GTPase activity; regulation of Rap protein signal transduction
Disease: Deafness, Autosomal Recessive 24
Research Articles on RDX
Similar Products
Product Notes
The RDX rdx (Catalog #AAA421449) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Radixin Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Radixin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem.1995 Oct 27;270(43): 25324-7. PMID: 7592691). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the RDX rdx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QARDETKKTQ ND. It is sometimes possible for the material contained within the vial of "Radixin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
