Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 1 (SPNS1) Standard Curve.)
Human Protein Spinster Homolog 1 (SPNS1) ELISA Kit | SPNS1 elisa kit
Human Protein Spinster Homolog 1 (SPNS1) ELISA Kit
Gene Names
SPNS1; LAT; nrs; SPIN1; SPINL; HSpin1; PP2030; SLC62A1
Reactivity
Human
Synonyms
Protein Spinster Homolog 1 (SPNS1); Human Protein Spinster Homolog 1 (SPNS1) ELISA Kit; SPNS1; FLJ38358; HSpin1; LAT; PP2030; SPIN1; SPINL; nrs; spinster homolog 1; spinster-like; SPNS1 elisa kit
Reactivity
Human
Specificity
Human Protein spinster homolog 1 (SPNS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPNS1. No significant cross-reactivity or interference between Human SPNS1 and analogues was observed.
Sequence Length
528
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 1 (SPNS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 1 (SPNS1) Standard Curve.)
Related Product Information for SPNS1 elisa kit
Description: This Human Protein spinster homolog 1 (SPNS1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPNS1 in samples. An antibody specific for SPNS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPNS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker.
Background: SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein spinster homolog 1 isoform 1
NCBI Official Synonym Full Names
sphingolipid transporter 1 (putative)
NCBI Official Symbol
SPNS1
NCBI Official Synonym Symbols
LAT; nrs; SPIN1; SPINL; HSpin1; PP2030; SLC62A1
NCBI Protein Information
protein spinster homolog 1
UniProt Protein Name
Protein spinster homolog 1
Protein Family
UniProt Gene Name
SPNS1
UniProt Synonym Gene Names
SPIN1
UniProt Entry Name
SPNS1_HUMAN
Uniprot Description
SPNS1: Sphingolipid transporter. May be involved in necrotic or autophagic cell death. Belongs to the major facilitator (TC 2.A.1) superfamily. Spinster (TC 2.A.1.49) family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: lysosomal membrane; mitochondrial inner membrane; integral to membrane; vesicle
Molecular Function: protein binding; sphingolipid transporter activity
Biological Process: lipid transport; locomotion; transmembrane transport
Research Articles on SPNS1
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Product Notes
The Human SPNS1 spns1 (Catalog #AAA9713996) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713996 ELISA Kit recognizes Human SPNS1. It is sometimes possible for the material contained within the vial of "Protein Spinster Homolog 1 (SPNS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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