Human DEAD Box Polypeptide 5 (DDX5) ELISA Kit | DDX5 elisa kit

Human DEAD Box Polypeptide 5 (DDX5) ELISA Kit

Cat. Num. AAA451151

• Gene Names: DDX5; p68; HLR1; G17P1; HUMP68
• Reactivity: Human
• Synonyms: DEAD Box Polypeptide 5 (DDX5); Human DEAD Box Polypeptide 5 (DDX5) ELISA Kit; G17P1; HLR1; HELR; HUMP68; p68; Asp-Glu-Ala-Asp Box Polypeptide 5; Probable ATP-dependent RNA helicase DDX5; DDX5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DDX5. No significant cross-reactivity or interference between DDX5 and analogues was observed.
• Sequence Length: 614

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DDX5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DDX5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DDX5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DDX5 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDX5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DDX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DDX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DDX5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1002341719
• NCBI GeneID: 1655
• NCBI Accession #: NP_001307524.1
• NCBI GenBank Nucleotide #: NM_001320595.1
• UniProt Accession #: P17844; O75681; Q53Y61; B4DLW8; B5BU21; D3DU32; E7ETL9
• Molecular Weight: 60,563 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX5 isoform a
• NCBI Official Synonym Full Names: DEAD-box helicase 5
• NCBI Official Symbol: DDX5
• NCBI Official Synonym Symbols: p68; HLR1; G17P1; HUMP68
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX5
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX5
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX5
• UniProt Synonym Gene Names: G17P1; HELR; HLR1

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.