Mouse Soluble Interleukin 6 Receptor ELISA Kit | sIL-6R elisa kit

Mouse Soluble Interleukin 6 Receptor ELISA Kit

Cat. Num. AAA760381

• Gene Names: IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• Reactivity: Mouse
• Synonyms: Soluble Interleukin 6 Receptor; Mouse Soluble Interleukin 6 Receptor ELISA Kit; sIL-6R; sIL-6R elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of sIL-6R. No significant cross-reactivity or interference between sIL-6R and analogues was observed.
• Sequence Length: 53

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 12.5-800pg/ml
• Sensitivity: 75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level sIL- 6R were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level sIL- 6R were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for sIL-6R elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- sIL-6R antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- sIL-6R antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the sIL-6R amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of sIL-6R can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 633863
• NCBI GeneID: 3570
• Molecular Weight: 40,237 Da
• NCBI Official Full Name: Soluble interleukin-6 receptor, partial
• NCBI Official Synonym Full Names: interleukin 6 receptor
• NCBI Official Symbol: IL6R
• NCBI Official Synonym Symbols: IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• NCBI Protein Information: interleukin-6 receptor subunit alpha
• UniProt Protein Name: Interleukin-6 receptor subunit alpha
• UniProt Gene Name: IL6R
• UniProt Synonym Gene Names: IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80
• UniProt Entry Name: IL6RA_HUMAN

NCBI Description

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]

Uniprot Description

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: apical plasma membrane; basolateral plasma membrane; extracellular region; extracellular space; interleukin-6 receptor complex; plasma membrane

Molecular Function: ciliary neurotrophic factor receptor activity; enzyme binding; interleukin-6 binding; interleukin-6 receptor activity; interleukin-6 receptor binding; protein binding; protein homodimerization activity

Biological Process: activation of NF-kappaB transcription factor; acute-phase response; cytokine and chemokine mediated signaling pathway; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; endocrine pancreas development; hepatic immune response; monocyte chemotaxis; negative regulation of collagen biosynthetic process; negative regulation of interleukin-8 production; neutrophil mediated immunity; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; positive regulation of MAPKKK cascade; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of tyrosine phosphorylation of Stat3 protein; response to cytokine stimulus

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus