Rabbit anti-Human SPAG16 Polyclonal Antibody | anti-SPAG16 antibody

Anti-SPAG16 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109354

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: SPAG16; Polyclonal Antibody; Anti-SPAG16 Antibody; Rabbit Polyclonal; SPAG16 Antibody; Rabbit PAb; Antigen Affinity Purified; sperm associated antigen 16; Anti-PF20 Antibody; Anti-WDR29 Antibody; anti-SPAG16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SPAG16
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SPAG16 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:500-1:2500
• Immunogen: E Coli-derived Human SPAG16 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human SPAG16 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SPAG16 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa Observed band size:70 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of SPAG16 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SPAG16 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-SPAG16 antibody

Sperm-associated antigen 16 (SPAG16), a sperm protein that is upregulated in reactive astrocytes in multiple sclerosis (MS) lesions, has been identified as a novel autoantibody target in MS. The SPAG16 gene encodes two major transcripts, one for the 71-kDa SPAG16L, which is the orthologue of the Chlamydomonas reinhardtii central apparatus protein PF20, and a smaller transcript, which codes for the 35-kDa SPAG16S nuclear protein that represents the C-terminus (exons 11-16) of SPAG16L. In sperm, SPAG16L is found in the central apparatus of the axoneme. SPAG16 is a critical structural component of motile cilia and flagella. In the eukaryotic unicellular algae Chlamydomonas, loss of gene function causes flagellar paralysis and prevents assembly of the "9 + 2" axoneme central pair. SPAG6 and SPAG16 are homologous in the mice to Chlamydomonas reinhardtii PF16 and PF20.

References

Zhang Z, et al. (2007) A heterozygous mutation disrupting the spag16 gene results in biochemical instability of central apparatus components of the human sperm axoneme. Biol Reprod 77 (5): 864-871.Zhang L, et al. (2017) Transcriptional regulation of human sperm-associated antigen 16 gene by s-sox5. BMC Mol Biol 18 (1): 2.Nagarkatti-Gude DR, et al. (2012) Genetic variation in spag16 regions encoding the wd40 repeats is not associated with reduced sperm motility and axonemal defects in a population of infertile males. BMC Urol 12 27.de Bock L, et al. (2016) Anti-spag16 antibodies in primary progressive multiple sclerosis are associated with an elevated progression index. Eur J Neurol 23 (4): 722-728.