Rabbit anti-Human SOX-4 Polyclonal Antibody | anti-SOX-4 antibody

Rabbit anti SOX-4 Polyclonal Antibody

Cat. Num. AAA460381

• Gene Names: SOX4; EVI16
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: SOX-4; Polyclonal Antibody; Rabbit anti SOX-4 Polyclonal Antibody; SRY (sex determining region Y)-box 4; anti-SOX-4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~94 kDa of SOX6 protein. It cross-reacts to human, mouse, rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 54

• Applicable Applications for anti-SOX-4 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the c-terminus (from 720AA-755aa) of human sox6 protein. This sequence is identical in human, mouse, rat, bovine, canis and chicken species.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-SOX-4 antibody

SOX6 gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. SOX6 protein is a transcriptional activator that is specifically expressed in the developing central nervous system and in the early stages of chondrogenesis in mouse embryos. It is found to be essential to both cartilage formation/chondrogenesis and obesity-related insulin resistance. The SOX6 protein is also detected in glioma patient sera and brain tissues.

References

Cohen-Barak, O., Hagiwara, N., Arlt, M.F., Horton, J.P. and Brilliant, M.H. Cloning, characterization and chromosome mapping of the human SOX6 gene. Gene 265 (1-2), 157-164 (2001)

NCBI and Uniprot Product Information

• NCBI GI #: 938230
• NCBI GeneID: 6659
• UniProt Accession #: Q06945
• Molecular Weight: 47,263 Da
• NCBI Official Full Name: SOX-4, partial
• NCBI Official Synonym Full Names: SRY-box 4
• NCBI Official Symbol: SOX4
• NCBI Official Synonym Symbols: EVI16
• NCBI Protein Information: transcription factor SOX-4
• UniProt Protein Name: Transcription factor SOX-4
• UniProt Gene Name: SOX4

NCBI Description

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX4: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; mitochondrion; nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; glial cell development; glial cell proliferation; glucose homeostasis; heart development; limb bud formation; negative regulation of cell proliferation; negative regulation of protein export from nucleus; negative regulation of protein ubiquitination; neural tube formation; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translation; positive regulation of Wnt receptor signaling pathway; pro-B cell differentiation; protein stabilization; regulation of protein stability; regulation of transcription, DNA-dependent; skeletal development; spinal cord development; spinal cord motor neuron differentiation; sympathetic nervous system development; T cell differentiation; Wnt receptor signaling pathway through beta-catenin