anti-Human SOX4 Polyclonal Antibody | anti-SOX4 antibody

Anti-SOX4 Antibody

Cat. Num. AAA177819

• Gene Names: SOX4; EVI16
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: SOX4; Polyclonal Antibody; Anti-SOX4 Antibody; Transcription factor SOX-4; Ecotropic viral integration site 16; EVI 16; EVI16; Sox4; SOX4_HUMAN; SRY (sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; SRY (sex determining region Y)-box 4; anti-SOX4 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 474

• Applicable Applications for anti-SOX4 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SOX4 (1-31aa MVQQTNNAENTEALLAGESSDSGAGLELGIA), identical to the related mouse sequence.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- SOX4 Picoband antibody, MBS177819, Western blottingAll lanes: Anti SOX4 (MBS177819) at 0.5ug/mlLane 1: MCF-7 Whole Cell Lysate at 40ugLane 2: 293T Whole Cell Lysate at 40ugPredicted bind size: 47KDObserved bind size: 47KD )

Related Product Information for anti-SOX4 antibody

Description: Rabbit IgG polyclonal antibody for Transcription factor SOX-4(SOX4) detection. Tested with WB in Human.

Background: Transcription factor SOX-4 is a protein that in humans is encoded by the SOX4 gene. This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). This protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

References

1. "Entrez Gene: SOX4 SRY (sex determining region Y)-box 4". 2. Critcher R, Stitson RN, Wade-Martins R, Easty DJ, Farr CJ (October 1998). "Assignment of Sox4 to mouse chromosome 13 bands A3-A5 by fluorescence in situ hybridization; refinement of the human SOX4 location to 6p22.3 and of SOX20 to chromosome 17p12.3". Cytogenet Cell Genet81 (3-4): 294-5. 3. Farr CJ, Easty DJ, Ragoussis J, Collignon J, Lovell-Badge R, Goodfellow PN (January 1994). "Characterization and mapping of the human SOX4 gene". Mamm Genome 4 (10): 577-84.

NCBI and Uniprot Product Information

• NCBI GI #: 4507163
• NCBI GeneID: 6659
• NCBI Accession #: NP_003098.1
• NCBI GenBank Nucleotide #: NM_003107.2
• UniProt Accession #: Q06945
• Molecular Weight: 47,263 Da
• NCBI Official Full Name: transcription factor SOX-4
• NCBI Official Synonym Full Names: SRY-box 4
• NCBI Official Symbol: SOX4
• NCBI Official Synonym Symbols: EVI16
• NCBI Protein Information: transcription factor SOX-4
• UniProt Protein Name: Transcription factor SOX-4
• Protein Family: Transcription factor
• UniProt Gene Name: SOX4
• UniProt Entry Name: SOX4_HUMAN

NCBI Description

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX4: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; mitochondrion; nucleoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity

Biological Process: DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endocrine pancreas development; glial cell development; glial cell proliferation; glucose homeostasis; heart development; limb bud formation; negative regulation of cell proliferation; negative regulation of protein export from nucleus; negative regulation of protein ubiquitination; neural tube formation; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translation; positive regulation of Wnt receptor signaling pathway; pro-B cell differentiation; protein stabilization; regulation of protein stability; regulation of transcription, DNA-dependent; skeletal development; somatic stem cell maintenance; spinal cord development; spinal cord motor neuron differentiation; sympathetic nervous system development; T cell differentiation; transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin