Western Blot (WB)
(Fig-1: Western blot analysis of IL-3RA. Anti-IL-3RA antibody (Clone: ABM25C1) was used at 6 ug/ml on hbrain lysate.)
Mouse anti-Human IL-3RA Monoclonal Antibody | anti-IL3RA antibody
Monoclonal Antibody to IL-3RA (Clone: ABM25C1)
Protein G Chromatography
Protein G Chromatography
Flow Cytometry: 0.5-1 ug/10^6
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig-1: Western blot analysis of IL-3RA. Anti-IL-3RA antibody (Clone: ABM25C1) was used at 6 ug/ml on hbrain lysate.)
Western Blot (WB)
(Fig-1: Western blot analysis of IL-3RA. Anti-IL-3RA antibody (Clone: ABM25C1) was used at 6 ug/ml on hbrain lysate.)
Flow Cytometry (FC/FACS)
(Fig-2: Cell surface flow analysis of human IL3-RA in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM25C1). Green represents isotype control; red represents anti-IL3 RA antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
Flow Cytometry (FC/FACS)
(Fig-2: Cell surface flow analysis of human IL3-RA in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM25C1). Green represents isotype control; red represents anti-IL3 RA antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
Uniprot Description
IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.2
Cellular Component: plasma membrane
Molecular Function: interleukin-3 receptor activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity
Biological Process: MAPKKK cascade
Research Articles on IL3RA
Similar Products
Product Notes
The IL3RA il3ra (Catalog #AAA668414) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to IL-3RA (Clone: ABM25C1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-3RA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). WB: 6-8 ug/ml Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the IL3RA il3ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-3RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
