Rabbit SOD Polyclonal Antibody | anti-SOD antibody

SOD (Mn) Antibody

Cat. Num. AAA8001996

• Gene Names: Sod2; MnSOD
• Reactivity: Human, Rat, Mouse, Bovine, Dog, Chicken, Fruit Fly, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, African Clawed Frog, Squirrel, Invertebrate, Sea Squirt
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA
• Purity: Protein A Purified
• Synonyms: SOD; Polyclonal Antibody; SOD (Mn) Antibody; Rabbit Anti-Rat SOD (Mn) Polyclonal; Manganese SOD Antibody; IPO B Antibody; Mn SOD Antibody; SOD2 Antibody; anti-SOD antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Bovine, Dog, Chicken, Fruit Fly, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, African Clawed Frog, Squirrel, Invertebrate, Sea Squirt
• Clonality: Polyclonal
• Specificity: Detects ~25kDa.
• Purity/Purification: Protein A Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 222

• Applicable Applications for anti-SOD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:5000; IHC: 1:100; ICC/IF: 1:120
• Immunogen: Rat Mn SOD
• Immunogen Species: Rat
• Conjugate: Unconjugated
• Cellular Localization: Mitochondrion, Mitochondrion Matrix
• Certificate of Analysis: 0.5 ug/ml of SPC-117 was sufficient for detection of Mn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.)

Western Blot (WB)

(Western blot analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody . Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.)

Related Product Information for anti-SOD antibody

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Product Categories/Family for anti-SOD antibody

Cancer; Oxidative Stress; Cell Signaling; Neuroscience; Neurodegeneration; Cardiovascular System

References

1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

NCBI and Uniprot Product Information

• NCBI GI #: 8394331
• NCBI GeneID: 24787
• NCBI Accession #: NP_058747.1
• NCBI GenBank Nucleotide #: NM_017051.2
• UniProt Accession #: P07895
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 2
• NCBI Official Symbol: Sod2
• NCBI Official Synonym Symbols: MnSOD
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• Protein Family: Sodium channel
• UniProt Gene Name: Sod2

NCBI Description

manganese superoxide dismutase; intramitochondrial free radical scavenging enzyme [RGD, Feb 2006]

Uniprot Description

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.